A highly sensitive and specific immunochromatographic test strip for detecting p,p′-DDA in seawater and shellfish

文献类型： 外文期刊

第一作者： Zhang, Teng

作者： Zhang, Teng;Yao, Jiale;Yang, Zhuan;Yao, Lunguang;Xing, Yunrui;Wang, Jucai;Chen, Linlin

作者机构：

关键词： p ' -DDA; Monoclonal antibody; Recombinant full-length antibody; Immunochromatographic strip; Recognition mechanism

期刊名称：FOOD CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.7 ）

ISSN： 0308-8146

年卷期： 2025 年 492 卷

页码：

收录情况： SCI

摘要： P,p '-DDA, similar to DDT, accumulates in the food chain and poses potential risks to human health. Herein, a novel sensitive and specific mAb-10B4D7 was screened and subsequently rAb-10B4D7 was expressed by genetic engineering. Utilizing rAb-10B4D7, an immunochromatographic test strip (ITS) was developed with visual limit of detection 12.5 mu g/L within 15 min, displaying no cross-reactivity with o,p '-DDT, p,p '-DDT, p,p '-DDD, and p,p ' DDE. Further, a linear relationship between log(concentration of p,p '-DDA) and the optical density ratio of test (T) line and control (C) line was established with limits of detection of 1.03 mu g/kg, recoveries of 79.3-106.8 %, and coefficients of variation below 10 %. Molecular docking simulations identified two critical residues (ArgH62, Phe-L98) forming three hydrogen bonds with p,p '-DDA, resulting in the high specificity and affinity of rAb10B4D7, with a KD of 5.81 x 10-9 mol/L. These results indicated that the ITS was suitable for monitoring p,p ' DDA and organochlorine residues in seawater and shellfish.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序