De Novo Transcriptome Analysis of the Lizard Fish (Saurida elongata): Novel Insights into Genes Related to Sex Differentiation

文献类型： 外文期刊

第一作者： Shan, Binbin

作者： Shan, Binbin;Wang, Liangming;Liu, Yan;Yang, Changping;Liu, Manting;Sun, Dianrong;Shan, Binbin;Wang, Liangming;Liu, Yan;Yang, Changping;Liu, Manting;Sun, Dianrong;Shan, Binbin;Wang, Liangming;Liu, Yan;Yang, Changping;Liu, Manting;Sun, Dianrong;Shan, Binbin;Liu, Yan;Yang, Changping;Sun, Dianrong;Huang, Pujiang

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关键词： demersal fish; synodontidae; RNA-seq; gene ontology; differentially expressed genes

期刊名称：APPLIED SCIENCES-BASEL （ 影响因子：2.838； 五年影响因子：2.921 ）

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年卷期： 2022 年 12 卷 22 期

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收录情况： SCI

摘要： Featured Application In our study, transcriptomes of different genders of lizardfish (Saurida elongata) were compared. Interesting findings about sex-related genes for putative future aquaculture applications are reported here. We provide a transcriptome dataset of S. elongata that will be valuable for further research into the reproductive biology of S. elongata and other teleost fishes. Among vertebrates, teleost fishes exhibit the largest array of sex-determining systems, resulting in many reproductive strategies. Screening these fish for sex-related genes could enhance our understanding of sexual differentiation. The lizardfish, Saurida elongata (Temminck & Schlegel, 1846), is a commercially important marine fish in tropical and subtropical seas of the northwest Pacific. However, little genomic information on S. elongata is available. In this study, the transcriptomes of three female and three male S. elongata were sequenced. A total of 49.19 million raw read pairs were generated. After identification and assembly, a total of 59,902 nonredundant unigenes were obtained with an N50 length of 2070 bp. Then, 38,016 unigenes (63.47% of the total) were successfully annotated through multiple public databases. A comparison of the unigenes of different sexes of S. elongata revealed that 22,507 unigenes (10,419 up-regulated in a female and 12,088 up-regulated in a male) were differentially expressed between sexes. Then, numerous candidate sex-related genes were identified, including dmrt2, dmrt4, foxl2, zps and starts. Furthermore, 23,941 simple sequence repeats (SSRs) were detected in SSR-containing sequences. This informative transcriptome analysis provides valuable data to increase the genomic resources of S. elongata.

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