Light-mediated activation of PpPSY enhances β-carotene accumulation in pear fruit peel

文献类型： 外文期刊

第一作者： Zhang, Li

作者： Zhang, Li;Du, Wei;Tu, Junfan;Zhu, Hongyan;Li, Xianming

作者机构：

关键词： light; pear; carotenoid; PSY; AGL8; LFY

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：4.8； 五年影响因子：5.7 ）

ISSN： 1664-462X

年卷期： 2025 年 16 卷

页码：

收录情况： SCI

摘要： Light is a key environmental factor that regulates fruit development and influences several important quality traits, including pericarp color. In pear fruits, carotenoids are the primary determinant of pericarp color. However, the molecular mechanisms underlying light-mediated carotenoid accumulation remain poorly understood. This study investigated the carotenoid contents in the peels of non-bagged (light-exposed) and bagged (shaded) pear fruits (Cuiguan, Pyrus pyrifolia) and revealed a significant differences in beta-carotene content between the two treatments. Transcriptome analysis revealed that the expression of phytoene synthase (PSY) was downregulated in bagged fruits, highlighting the regulatory role of PSY in carotenoid metabolism. To further validate this, we transiently overexpressed PSY, which resulted in a marked increase in beta-carotene levels at the injection site. Conversely, transient silencing of PSY led to a significant reduction in the beta-carotene content, confirming the pivotal role of PSY in regulating beta-carotene accumulation. Promoter analysis revealed that agamous-like 8 (AGL8) directly binds to the PSY promoter to activate its transcription. Protein-protein interaction assays demonstrated that AGL8 interacts with LEAFY (LFY), thereby increasing PSY expression. In conclusion, the AGL8-LFY complex coactivates PSY expression, regulating beta-carotene accumulation in pear fruit. This study provides new insights into the regulatory network governing fruit peel coloration, with potential applications for cultivation strategies to improve fruit quality.

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