CLONING AND EXPRESSION ANALYSIS OF TWO UDP-GLUCOSYLTRANSFERASES GENES IN WHEAT

文献类型： 外文期刊

第一作者： Lin, F. Y.

作者： Lin, F. Y.;Shi, J. R.;Lu, Q. X.;Xu, J. H.;Yang, H. Y.

作者机构：

关键词： wheat;Glycosyltransferases;gene expression

期刊名称：CEREAL RESEARCH COMMUNICATIONS （ 影响因子：0.85； 五年影响因子：1.067 ）

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收录情况： SCI

摘要： Glycosyltransferases (GTs) play important roles in plant stress responses by glycosylating hormones and secondary metabolites. UDP-glucosyltransferases (UGTs), which belong to family 1 of GTs, use UDP-glucuronic acid in animals, UDP-glucose, UDP-galactose and UDP- rhamnose in plant as sugar donors. Deoxynivalenol (DON) is one of trichothecenes produced by Fusarium graminearum when infecting grains. DON is not only harmful to human and animal's health, but also acts as a virulence factor during fungalpathogenesis. \nArabidopsis, a UDP-glycosyltransferase (UGT73C5) is able to detoxify deoxynivalenol by glycosylation. In transgenic Arabidopsis, the corresponding gene, DOGT1, could increase DON resistance significantly. In order to study the sequence and expression profile of the homologous gene in wheat, two UGTs were isolated from wheat by reverse transcriptase-polymerase chain reaction and named as TaUGTl and TaUGT2, respectively. The two genes both had an intron and had 91% and 90% sequence similarities on nucleic acid and deduced protein sequence level. Analysis of conserved domain revealed that the two cDNA belonged to UDP-glucoronosyl and UDP-glucosyl transferase genes. The two protein contained PSPG (Putative Secondary Plant Glycosyltransferase) domain. Real-time PCR were employed to detect the genes expression profiles in wheat under different stress conditions. In Fusarium graminearum infected young spikes, TaUGT2 was induced while TaUGTl was repressed. The two genes were down-regulated under lower PEG concentration, but were up-regulated under higher NaCl concentration. In conclustion, TaUGT2 may play a role in Fusarium head blight resistance, and the two genes may be both involved the responses of wheat to salt stress.

分类号： S5

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