Preparation of a cattle bone collagen peptide-calcium chelate by the ultrasound method and its structural characterization, stability analysis, and bioactivity on MC3T3-E1 cells

文献类型： 外文期刊

第一作者： Zhang, Hongru

作者： Zhang, Hongru;Qi, Liwei;Guo, Yujie;Liu, Jiqian;Zhang, Chunhui;Zhang, Hongru;Richel, Aurore;Wang, Xiaodan;Liu, Chengjiang;Xu, Yang

作者机构：

期刊名称：FOOD & FUNCTION （ 影响因子：6.317； 五年影响因子：6.375 ）

ISSN： 2042-6496

年卷期：

页码：

收录情况： SCI

摘要： This study was designed to prepare a cattle bone-derived collagen peptide-calcium chelate by the ultrasound method (CP-Ca-US), and its structure, stability, and bioactivity on MC3T3-E1 cells were characterized. Single-factor experiments optimized the preparation conditions: ultrasound power 90 W, ultrasound time 40 min, CaCl2/peptides ratio 1/2, pH 7. Under these conditions, the calcium-chelating ability reached 39.48 mu g mg(-1). The result of Fourier transform-infrared spectroscopy indicated that carboxyl oxygen and amino nitrogen atoms were chelation sites. Morphological analysis indicated that CP-Ca-US was characterized by a porous surface and large particles. Stability analysis demonstrated that CP-Ca-US was stable in the thermal environment and under intestinal digestion. CP-Ca-US showed more stability in gastric juice than the chelate prepared by the hydrothermal method. Cell experiments indicated that CP-Ca-US increased osteoblast proliferation (proliferation rate 153% at a concentration of 300 mu g mL(-1)) and altered the cell cycle. Significantly, CP-Ca-US enhanced calcium absorption by interacting with calcium-sensing receptors and promoted the mineralization of MC3T3-E1 cells. This study provides the scientific basis for applying the ultrasound method to prepare peptide-calcium chelates and clarifies the positive role of chelates in bone building.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序