In vitro effects of structurally diverse low molecular weight chondroitin sulfates on gut microbiota and metabolome

文献类型： 外文期刊

第一作者： Wang, Kangyu

作者： Wang, Kangyu;Liu, Yue;Guo, Yujie;Zhang, Chunhui;Zhang, Chunhui

作者机构：

关键词： Low molecular weight chondroitin sulfate; Structure; Gut microbiota; Metabolism

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 310 卷

页码：

收录情况： SCI

摘要： In this study, low molecular weight chondroitin sulfates (LMCSs) with different structures, named LMCSO, LMCSD, and LMCSH, were prepared by oxidative degradation, deamidation cleavage, and hydrothermal depolymerization, respectively. In vitro fermentation modeling was used to study the effects of CS and LMCSs on gut microbiota and metabolite composition. The degree of carbohydrate metabolism was in the order of CS > LMCSH > LMCSO > LMCSD. Significantly, GlcA in chondroitin-6-sulfate (CSC) was more readily utilized by gut microbiota during fermentation, and this trend was more pronounced in LMCSs. The LMCSs group notably increased microbial richness and evenness, especially in the LMCSD group. Bacteroides fragilis was identified as a potential primary degrader of CS and LMCSs through species-level analysis. The abundance of Escherichia-Shigella was reduced by LMCSs, and short-chain fatty acids production was enhanced, particularly by LMCSO, while the production of beneficial metabolites such as N-acetyl-D-Glucosamine 6-Phosphate (GlcNAc-6P), lactate, and progesterone was stimulated. Among these, the metabolism of the key metabolite GlcNAc-6P was significantly and positively correlated with the abundance of Bacteroides, Clostridium_sensu_stricto_1, and Parabacteroides. Exploring the mechanisms by which gut microbiota metabolize LMCSs with different structures can provide theoretical support for the targeted preparation of LMCSs that modulate the gut microbiota.

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