BSA-seq and genetic mapping identified candidate genes for branching habit in peanut

文献类型： 外文期刊

第一作者： Pan, Jiaowen

作者： Pan, Jiaowen;Zhou, Ximeng;Ahmad, Naveed;Zhang, Kun;Zhao, Huiling;Li, Changsheng;Li, Aiqin;Zhang, Xianying;Ma, Jing;Li, Xiaojie;Tian, Ruizheng;Wang, Xingjun;Zhao, Chuanzhi;Zhou, Ximeng;Zhao, Huiling;Ma, Jing;Li, Xiaojie;Ma, Changle;Wang, Xingjun;Zhao, Chuanzhi;Zhang, Kun;Tang, Ronghua;Jiang, Jing;He, Liangqiong;Tian, Mengdi;Pandey, Manish K.;Varshney, Rajeev K.

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 12 期

页码：

收录情况： SCI

摘要： Key Message The candidate gene AhLBA1 controlling lateral branch angel of peanut was fine-mapped to a 136.65-kb physical region on chromosome 15 using the BSA-seq and QTL mapping. Lateral branch angel (LBA) is an important plant architecture trait of peanut, which plays key role in lodging, peg soil penetration and pod yield. However, there are few reports of fine mapping and quantitative trait loci (QTLs)/cloned genes for LBA in peanut. In this project, a mapping population was constructed using a spreading variety Tifrunner and the erect variety Fuhuasheng. Through bulked segregant analysis sequencing (BSA-seq), a major gene related to LBA, named as AhLBA1, was preliminarily mapped at the region of Chr.15: 150-160 Mb. Then, using traditional QTL approach, AhLBA1 was narrowed to a 1.12 cM region, corresponding to a 136.65-kb physical interval of the reference genome. Of the nine genes housed in this region, three of them were involved in hormone metabolism and regulation, including one "F-box protein" and two "2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase (2OG oxygenase)" encoding genes. In addition, we found that the level of some classes of cytokinin (CK), auxin and ethylene showed significant differences between spreading and erect peanuts at the junction of main stem and lateral branch. These findings will aid further elucidation of the genetic mechanism of LBA in peanut and facilitating marker-assisted selection (MAS) in the future breeding program.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序