Chemical gene synthesis: strategies, softwares, error corrections, and applications

文献类型： 外文期刊

第一作者： Xiong, Ai-Sheng

作者： Xiong, Ai-Sheng;Peng, Ri-He;Zhuang, Jing;Gao, Feng;Yao, Quan-Hong;Cheng, Zong-Ming;Li, Yi

作者机构：

关键词： chemical gene synthesis;PCR;primer design;error correction;application;biotechnology;HIGH-LEVEL EXPRESSION;SITE-DIRECTED MUTAGENESIS;OVERLAP EXTENSION PCR;HETEROLOGOUS PROTEIN-PRODUCTION;HEPATITIS-B-VIRUS;FLANKING HOMOLOGY REGIONS;POLYMERASE-CHAIN-REACTION;IN-VITRO RECOMBINATION;SYNONYMOUS CODON USAGE;TRANSGENIC RICE PLANTS

期刊名称：FEMS MICROBIOLOGY REVIEWS （ 影响因子：16.408； 五年影响因子：18.835 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Chemical synthesis of DNA sequences provides a powerful tool for modifying genes and for studying gene structure, expression and function. Modified genes and consequently protein/enzymes can bridge genomics and proteomics research or facilitate commercial applications of gene and protein technologies. In this review, we will summarize various strategies, designing softwares and error correction methods for chemical gene synthesis, particularly for the synthesis and assembly of long DNA molecules based on polymerase cycling assembly. Also, we will briefly discuss some of the major applications of chemical synthesis of DNA sequences in basic research and applied areas.

分类号： Q93

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