Unveiling the hidden role of the interaction between CD36 and FcγRIIb: implications for autoimmune disorders

文献类型： 外文期刊

第一作者： He, Chenfei

作者： He, Chenfei;Hua, Guoying;Li, Shuijie;Li, Shuijie;He, Chenfei;Liu, Yong

作者机构：

关键词： Apoptotic cell; Autoreactive B cell; Fc gamma RIIb; Germinal center; Scavenger receptor CD36

期刊名称：CELLULAR & MOLECULAR BIOLOGY LETTERS （ 影响因子：8.3； 五年影响因子：7.2 ）

ISSN： 1425-8153

年卷期： 2024 年 29 卷 1 期

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收录情况： SCI

摘要： Background The role of the scavenger receptor CD36 in cell metabolism and the immune response has been investigated mainly in macrophages, dendritic cells, and T cells. However, its involvement in B cells has not been comprehensively examined.Methods To investigate the function of CD36 in B cells, we exposed Cd36 fl/fl MB1 cre mice, which lack CD36 specifically in B cells, to apoptotic cells to trigger an autoimmune response. To validate the proteins that interact with CD36 in primary B cells, we conducted mass spectrometry analysis following anti-CD36 immunoprecipitation. Immunofluorescence and co-immunoprecipitation were used to confirm the protein interactions.Results The data revealed that mice lacking CD36 in B cells exhibited a reduction in germinal center B cells and anti-DNA antibodies in vivo. Mass spectrometry analysis identified 30 potential candidates that potentially interact with CD36. Furthermore, the interaction between CD36 and the inhibitory Fc receptor Fc gamma RIIb was first discovered by mass spectrometry and confirmed through immunofluorescence and co-immunoprecipitation techniques. Finally, deletion of Fc gamma RIIb in mice led to decreased expression of CD36 in marginal zone B cells, germinal center B cells, and plasma cells.Conclusions Our data indicate that CD36 in B cells is a critical regulator of autoimmunity. The interaction of CD36-Fc gamma RIIb has the potential to serve as a therapeutic target for the treatment of autoimmune disorders.

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