An evolutionarily conserved C4HC3-type E3 ligase regulates plant broad-spectrum resistance against pathogens

文献类型： 外文期刊

第一作者： Fu, Shuai

作者： Fu, Shuai;Wang, Kun;Ma, Tingting;Liang, Yan;Ma, Zhonghua;Wu, Jianxiang;Xu, Yi;Zhou, Xueping;Ma, Tingting;Zhou, Xueping;Xu, Yi

作者机构：

期刊名称：PLANT CELL （ 影响因子：12.085； 五年影响因子：12.796 ）

ISSN： 1040-4651

年卷期： 2022 年 34 卷 5 期

页码：

收录情况： SCI

摘要： A microtubule-associated E3 ligase ubiquitinates and promotes degradation of a serine hydroxymethyltrasferase, resulting in broad-spectrum resistance to pathogens. Deployment of broad-spectrum disease resistance against multiple pathogen species is an efficient way to control plant diseases. Here, we identify a Microtubule-associated C4HC3-type E3 Ligase (MEL) in both Nicotiana benthamiana and Oryza sativa, and show that it is able to integrate and initiate a series of host immune signaling, conferring broad-spectrum resistance to viral, fungal, and bacterial pathogens. We demonstrate that MEL forms homodimer through intermolecular disulfide bonds between its cysteine residues in the SWIM domain, and interacts with its substrate serine hydroxymethyltrasferase 1 (SHMT1) through the Y phi NL motif. Ubiquitin ligase activity, homodimerization and Y phi NL motif are indispensable for MEL to regulate plant immunity by mediating SHMT1 degradation through the 26S proteasome pathway. Our findings provide a fundamental basis for utilizing the MEL-SHMT1 module to generate broad-spectrum-resistant rice to global destructive pathogens including rice stripe virus, Magnaporthe oryzae, and Xanthomonas oryzae pv. oryzae.

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