Effect of dietary supplementation with sanguinarine on meat quality and lipid metabolism of broilers

文献类型： 外文期刊

第一作者： Su, Yue

作者： Su, Yue;Wu, Zhiyong;Dai, Wanwan;Zeng, Jianguo;Huang, Peng;Zhang, Yan;Zeng, Jianguo

作者机构：

关键词： broiler; Sanguinarine; meat quality; lipid metabolism; gut microbiota

期刊名称：POULTRY SCIENCE （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 8 期

页码：

收录情况： SCI

摘要： Dietary Macleaya cordata extract ( MCE ) can improve the meat quality of poultry. However, the speci fic mechanism by which MCE regulates the meat quality has not been clari fied yet. Sanguinarine ( SAN ) is one of the important natural active components in MCE. Our study aims to explore the regulatory mechanism of dietary SAN supplementation on meat quality through transcriptomic and gut microbiome analysis, thereby providing a basis for regularing meat quality with MCE. 240 1 -day -old broilers were divided into 4 groups according to different doses of SAN (0, 0.225, 0.75, and 2.25 mg/kg). The results indicated that SAN signi ficantly improve the physicochemical quality indicators of breast and thigh muscle in broilers, improved the serum biochemical indexes. Through transcriptome sequencing analysis of the liver and ileum tissues of broilers, we found that the differentially expressed genes induced by SAN were mainly enriched in lipid metabolism, which were related to the peroxisome proliferator-activated receptor ( PPAR ) pathway. It recon firmed that SAN can regulate lipid metabolism in the body by promoting the expression of genes related to cholesterol metabolism, fatty acid transport and oxidation by RT-PCR, this ultimately affects the physicochemical quality of muscle. Additionally, through 16S rRNA sequencing analysis, we found that dietary addition of SAN increased the relative abundance of Bacteroides, Lactobacillus and unclassified_f_Lachnospiraceae , while decreased the relative abundance of Alistipes in ceca. To further investigate the impact of gut microbiota on lipid metabolism, we conducted a correlation analysis of PPAR pathway factor expression in cecum tissue and micro flora structure. The results showed that Bacteroides exhibited a positive correlation with the expression of most genes in the PPAR signaling pathway. Unclassi fied_f__Lach- nospiraceae is positively correlated with PPAR g, Cytochrome P450 family 7 subfamily A member 1 ( CYP7A1 ) and Acyl-CoA synthetase long -chain family member 5 ( ACSL5 ). In conclusion, dietary addition of SAN can promote the genes expression of the PPAR pathway, target the regulation of intestinal micro flora structure and abundance and regulate lipid metabolism, thereby improving meat quality of broilers.

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