Molecular evolution and functional modification of plant miRNAs with CRISPR

文献类型： 外文期刊

第一作者： Deng, Fenglin

作者： Deng, Fenglin;Zeng, Fanrong;Cheng, Jianhui;Jiang, Wei;Shen, Qiufang;Abbas, Asad;Chen, Guang;Shah, Adnan Noor;Holford, Paul;Chen, Zhong-Hua;Tanveer, Mohsin;Zhang, Dabing;Zhang, Dabing;Chen, Zhong-Hua

作者机构：

期刊名称：TRENDS IN PLANT SCIENCE （ 影响因子：22.012； 五年影响因子：23.116 ）

ISSN： 1360-1385

年卷期： 2022 年 27 卷 9 期

页码：

收录情况： SCI

摘要： Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序