A Novel Polymer Nanoparticle Polydimethyl Diallyl Ammonium Chloride as An Adjuvant Enhances the Immune Response of SARS-CoV-2 Subunit Vaccine

文献类型： 外文期刊

第一作者： Ren, Lili

作者： Ren, Lili;Ouyang, Chengcheng;Zhao, Shuqing;Zheng, Qiqi;Guo, Weilu;Zhang, Wei;Zhao, Shuqing;Zheng, Qiqi;Guo, Weilu;Fan, Baochao;Zhou, Jinzhu;Hu, Mi;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin;Fan, Baochao;Li, Jizong;Li, Bin

作者机构：

关键词： adjuvant; polydimethyl diallyl ammonium chloride; SARS-CoV-2; subunit vaccine

期刊名称：ADVANCED HEALTHCARE MATERIALS （ 影响因子：10.0； 五年影响因子：10.6 ）

ISSN： 2192-2640

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The Coronavirus Disease 2019 (COVID-19) pandemic caused by SARS-CoV-2 has a significant impact on global health and the economy. It has underscored the urgent need for a stable, easily produced and effective vaccine. This study presents a novel approach using SARS-CoV-2 spike (S) protein-conjugated nanoparticles (NPs) in combination with cyclic GMP-AMP (cGAMP) (S-NPs-cGAMP) as a subunit vaccine. When mice are immunized, the antiserum of S-NPs-cGAMP group exhibits a 16-fold increase in neutralizing activity against a pseudovirus, compared to S protein group. Additionally, S-NPs-cGAMP induces even higher levels of neutralizing antibodies. Remarkably, the vaccine also triggers a robust humoral immune response, as evidenced by a notable elevation in virus-specific IgG and IgM antibodies. Furthermore, after 42 days of immunization, there is an observed increase in specific immune cell populations in the spleen. CD3+CD4+ and CD3+CD8+T lymphocytes, as well as B220+CD19+ and CD3-CD49b+ NK lymphocytes, show an upward trend, indicating a positive cellular immune response. Moreover, the S-NPs-cGAMP demonstrates promising results against the Delta strain and exhibits good cross-neutralization potential against other variants. These findings suggest that pDMDAAC NPs is potential adjuvant and could serve as a versatile platform for future vaccine development. The study develops a novel subunit vaccine (S-NPs-cGAMP) delivered by the polymer polydimethyl diallyl ammonium chloride (pDMDAAC) with SARS-CoV-2 S protein combined with cGAMP adjuvant. The S-NPs-cGAMP induces more robust immune response in mice, compared to SARS-CoV-2 S protein. These findings suggest that pDMDAAC has the immunopotentiating properties and could be a potential adjuvant. image

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序