Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes

文献类型： 外文期刊

第一作者： Yang, Lei

作者： Yang, Lei;Xing, Binglin;Li, Fen;Wang, Li Kui;Yuan, Linlin;Mbuji, Amosi Leonard;Wu, Shaoying;Mbuji, Amosi Leonard;Peng, Zhengqiang;Malhat, Farag

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关键词： Spodoptera frugiperda; Resistance; Full-length transcriptome; Detoxification gene

期刊名称：PEERJ （ 影响因子：2.984； 五年影响因子：3.369 ）

ISSN： 2167-8359

年卷期： 2021 年 9 卷

页码：

收录情况： SCI

摘要： Background: Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce. Methods: In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes. Results: A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified.

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