Identifying the role of cellulase gene CsCEL20 upon the infection of Xanthomonas citri subsp. citri in citrus

文献类型： 外文期刊

第一作者： Li, Yi

作者： Li, Yi;Lou, Huijie;Su, Hanying;Hao, Chenxing;Luo, Jianming;Cai, Nan;Jin, Yan;Deng, Ziniu;Cao, Yunlin;Ma, Xianfeng;Li, Yi;Lou, Huijie;Su, Hanying;Hao, Chenxing;Luo, Jianming;Cai, Nan;Jin, Yan;Deng, Ziniu;Cao, Yunlin;Ma, Xianfeng;Fu, Hongyan;Han, Jian;Deng, Ziniu

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关键词： Citrus Canker; Cellulase; Endoglucanase; CsLOB1; CsCEL20

期刊名称：MOLECULAR BREEDING （ 影响因子：3.0； 五年影响因子：3.0 ）

ISSN： 1380-3743

年卷期： 2025 年 45 卷 1 期

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收录情况： SCI

摘要： Citrus canker is a devastating disease caused by Xanthomonas citri subsp. citri (Xcc), which secretes the effector PthA4 into host plants to trigger transcription of the susceptibility gene CsLOB1, resulting in pustule formation. However, the molecular mechanism underlying CsLOB1-mediated susceptibility to Xcc remains elusive. This study identified CsCEL20 as a target gene positively regulated by CsLOB1. Cell expansion and cell wall degradation were observed in sweet orange leaves after Xcc infection. A total of 69 cellulase genes were retrieved within the Citrus sinensis genome, comprising 40 endoglucanase genes and 29 glucosidase genes. Transcriptomic analysis revealed that expression levels of CsCEL8, CsCEL9, CsCEL20, and CsCEL26 were induced by Xcc invasion in sweet orange leaves, but not in the resistant genotype Citron C-05. Among them, CsCEL20 exhibited the highest expression level, with an over 430-fold increase following Xcc infection. Additionally, RT-qPCR analysis confirmed that CsCEL20 expression was induced in susceptible genotypes (Sweet orange, Danna citron, Lemon) upon Xcc invasion, but not in resistant genotypes (Citron C-05, Aiguo citron, American citron). A Single-Nucleotide Polymorphism (SNP) at -423 bp was identified in the CEL20 promoters and exhibits a difference between eight susceptible citrus genotypes and three resistant ones. Moreover, CsCEL20 expression was upregulated in CsLOB1-overexpression transgenic lines compared to the wild type. Dual-luciferase reporter assays indicated that CsLOB1 can target the -505 bp to -168 bp region of CsCEL20 promoter to trans-activate its expression. These findings suggest that CsCEL20 may function as a candidate gene for citrus canker development and may be a promising target for biotechnological breeding of Xcc-resistant citrus genotypes.

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