Engineering tropane alkaloid production and glyphosate resistance by overexpressing AbCaM1 and G2-EPSPS in Atropa belladonna

文献类型： 外文期刊

第一作者： Zhang, Qiaozhuo

作者： Zhang, Qiaozhuo;Liang, Mengjiao;Zeng, Junlan;Yang, Chunxian;Liao, Zhihua;Qin, Jianbo;Liao, Zhihua;Qiang, Wei;Lan, Xiaozhong;Chen, Min;Lin, Min

作者机构：

关键词： A tropa belladonna; Calmodulin; Genetic engineering; G2-EPSPS; Glyphosate resistance; Transgenic homozygous lines; Tropane alkaloids

期刊名称：METABOLIC ENGINEERING （ 影响因子：8.4； 五年影响因子：8.7 ）

ISSN： 1096-7176

年卷期： 2022 年 72 卷

页码：

收录情况： SCI

摘要： Atropa belladonna is an important industrial crop for producing anticholinergic tropane alkaloids (TAs). Using glyphosate as selection pressure, transgenic homozygous plants of A. belladonna are generated, in which a novel calmodulin gene (AbCaM1) and a reported EPSPS gene (G2-EPSPS) are co-overexpressed. AbCaM1 is highly expressed in secondary roots of A. belladonna and has calcium-binding activity. Three transgenic homozygous lines were generated and their glyphosate tolerance and TAs' production were evaluated in the field. Transgenic homozygous lines produced TAs at much higher levels than wild-type plants. In the leaves of T2GC02, T2GC05, and T2GC06, the hyoscyamine content was 8.95-, 10.61-, and 9.96 mg/g DW, the scopolamine content was 1.34-, 1.50-and 0.86 mg/g DW, respectively. Wild-type plants of A. belladonna produced hyoscyamine and scopolamine respectively at the levels of 2.45 mg/g DW and 0.30 mg/g DW in leaves. Gene expression analysis indicated that AbCaM1 significantly up-regulated seven key TA biosynthesis genes. Transgenic homozygous lines could tolerate a commercial recommended dose of glyphosate in the field. In summary, new varieties of A. belladonna not only produce pharmaceutical TAs at high levels but tolerate glyphosate, facilitating industrial production of TAs and weed management at a much lower cost.

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