Insights into production and consumption processes of nitrous oxide emitted from soilless culture systems by dual isotopocule plot and functional genes

文献类型： 外文期刊

第一作者： Lin, Wei

作者： Lin, Wei;Zhou, Wanlai;Yang, Rui;Zhang, Dongdong;Wang, Hong;Li, Yujia;Qi, Zhiyong;Li, QiaoZhen;Li, Yuzhong;Li, QiaoZhen;Li, Yuzhong

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关键词： Nitrification; Denitrification; N2O emission; N2O reduction; Soilless substrate

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：10.753； 五年影响因子：10.237 ）

ISSN： 0048-9697

年卷期： 2023 年 856 卷

页码：

收录情况： SCI

摘要： Soilless culture systems (SCS) play an increasing role in greenhouse vegetable production. In the SCS, soilless sub-strates serve as the major substitute for soil, supplying nutrients to plants but releasing greenhouse gases into the at-mosphere. Remarkably, there is a serious problem of N2O emission due to excessive input of N fertilizer. However, the microbial processes of N2O production and consumption in soilless substrates have been rarely studied resulting in difficultly interpreting for its global warming potential. Therefore, these pathways from two classic soilless sub-strates under two irrigation patterns were investigated by stable isotope technology combined with qPCR analysis in present study. The results according to the dual isotopocule plot of delta 15NSP vs. delta 18O showed that the mean contribution of denitrification and the mean extent of N2O reduction of case i (Reduction-Mixing) were 26.2 and 81.2 % for the treatment of peat based substrate under drip irrigation (PD), 47.7 and 70.3 % for the treatment of coir substrate under drip irrigation (CD), 29.0 and 80.8 % for the treatment of peat based substrate under tidal irrigation (PT), and 50.8 and 47.4 % for the treatment of coir substrate under tidal irrigation (CT). These results were also further con-firmed by the abundance of major functional genes including AOA amoA, nirK and nosZ. Altogether, N2O emission and its microbial processes are determined by substrate types instead of irrigation patterns. For detail, denitrification dom-inated in the peat based substrate and nitrification dominated in the coir substrate. Compared to the coir substrate, the peat based substrate had higher abundance of functional genes and stronger denitrification and thus generated more N2O. For the two soilless substrates, moreover, the microbiome replaced the mineral N content as the limiting factor for N2O emission. In the SCS, in summary, the two soilless substrates play an important role in tomato growth, but might suffer from inorganic nutrient surplus and microbial shortage. More importantly, the combined analysis of N2O isotopocule deltas and functional genes is a robust tool and provides reliable conclusions for clarifying the microbial processes of N2O production and consumption, thus it is also recommended for use in environments other than soilless substrates.

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