Molecular Cloning and Expression Analysis of the Myostatin Gene in Sea Perch (Lateolabrax japonicus)

文献类型： 外文期刊

第一作者： Ye, Han-Qing

作者： Ye, Han-Qing;Chen, Song-Lin;Sha, Zhen-Xia;Liu, Yang

作者机构：

关键词： embryonic stem cell;gene;gene expression;Lateolabrax japonicus;myostatin;sea perch

期刊名称：MARINE BIOTECHNOLOGY （ 影响因子：3.619； 五年影响因子：3.322 ）

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收录情况： SCI

摘要： Myostatin (MSTN) is a member of the transforming growth factor-beta (TGF-beta) superfamily that functions as a negative regulator of skeletal muscle development and growth in mammals. However, few reports are available about the structure and functionof MSTN in teleost. Here, the MSTN gene was cloned from sea perch (Lateolabrax japonicus) by homology cloning and genomic walking. In the 4873-bp genomic sequence, three exons, two introns, and 5' and 3' flanking sequences were identified. The sea perchMSTN gene encodes a 374-amino acid protein, including a signal peptide, conserved cysteine residues, and a RXXR proteolytic cleavage domain. Expression analysis of MSTN revealed that MSTN was highly expressed in eyes, brain, and muscle; intermediately in intestine; and weakly in gill, spleen, liver, and heart. It was demonstrated that MSTN mRNA was highly expressed in embryonic stem cell line (LJES1), but it was undetectable in several types of somatic cell lines from sea perch, including fibroblast-like cell, epithelioid cell, and lymphocyte-like cell. Further, it was demonstrated that the 5' flanking region of the MSTN gene can drive the expression of green fluorescent protein (GFP) reporter gene in LJES1 cells and transgenic zebrafish (Danio rerio). This is the first report on the expression profile of MSTN gene in various types of cell cultures.

分类号： Q81

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