Sequential pretreatment with hydroxyl radical and manganese peroxidase for the efficient enzymatic saccharification of corn stover

文献类型： 外文期刊

第一作者： Zhou, Man

作者： Zhou, Man;Wang, Yaru;Wang, Yuan;Tu, Tao;Zhang, Jie;Wang, Xiaolu;Huang, Huoqing;Yao, Bin;Luo, Huiying;Qin, Xing;Zhang, Guijie

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关键词： Hydroxyl radical; Manganese peroxidase; Pretreatment; Enzymatic saccharification; Corn stover

期刊名称：BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS （ 影响因子：4.6； 五年影响因子：4.6 ）

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年卷期： 2024 年 17 卷 1 期

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收录情况： SCI

摘要： BackgroundWhite rot fungi produce various reactive oxygen species and ligninolytic enzymes for lignocellulose deconstruction. However, their interactions during the deconstruction of lignocellulosic structural barriers for efficient enzymatic saccharification remain unclear.ResultsHerein, the extracellular enzyme activities and secretomic analysis revealed the sequential expression of hydroxyl radical (& sdot;OH) and manganese peroxidases (MnPs) for lignocellulose deconstruction by the white rot fungus Irpex lacteus. Subsequently, in vitro functional studies found that & sdot;OH possessed the ability to disrupt the smooth surface structure of corn stover, resulting in increased enzymatic saccharification and cellulose accessibility. Purified recombinant MnPs from I. lacteus were able to cleave the beta-O-4 bond in phenolic and non-phenolic lignin model dimers without the help of any mediators. Furthermore, the sequential pretreatment of corn stover with & sdot;OH and MnP exhibited significant synergistic effects, increasing enzymatic saccharification and cellulose accessibility by 2.9-fold and 1.8-fold, respectively.ConclusionsThese results proved for the first time the synergistic effects of & sdot;OH and MnP pretreatment in improving the enzymatic saccharification and cellulose accessibility of corn stover. These findings also demonstrated the potential application of & sdot;OH and MnP pretreatment for the efficient enzymatic saccharification of corn stover.

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