SNMP1 is critical for sensitive detection of the desert locust aromatic courtship inhibition pheromone phenylacetonitrile

文献类型： 外文期刊

第一作者： Lehmann, Joris

作者： Lehmann, Joris;Khosravian, Maryam;Cassau, Sina;Libnow, Johanna S.;Fleischer, Joerg;Krieger, Juergen;Guenzel, Yannick;Kraus, Susanne;Couzin-Fuchs, Einat;Guenzel, Yannick;Couzin-Fuchs, Einat;Guenzel, Yannick;Couzin-Fuchs, Einat;Guenzel, Yannick;Chang, Hetan;Hansson, Bill S.;Chang, Hetan;Breer, Heinz

作者机构：

关键词： Insect olfaction; Sensory neuron membrane protein 1; Schistocerca gregaria; Pheromone communication; Antenna; Antennal lobe

期刊名称：BMC BIOLOGY （ 影响因子：4.5； 五年影响因子：5.4 ）

ISSN：

年卷期： 2024 年 22 卷 1 期

页码：

收录情况： SCI

摘要： Background Accurate detection of pheromones is crucial for chemical communication and reproduction in insects. In holometabolous flies and moths, the sensory neuron membrane protein 1 (SNMP1) is essential for detecting long-chain aliphatic pheromones by olfactory neurons. However, its function in hemimetabolous insects and its role for detecting pheromones of a different chemical nature remain elusive. Therefore, we investigated the relevance of SNMP1 for pheromone detection in a hemimetabolous insect pest of considerable economic importance, the desert locust Schistocerca gregaria, which moreover employs the aromatic pheromone phenylacetonitrile (PAN) to govern reproductive behaviors. Results Employing CRISPR/Cas-mediated gene editing, a mutant locust line lacking functional SNMP1 was established. In electroantennography experiments and single sensillum recordings, we found significantly decreased electrical responses to PAN in SNMP1-deficient (SNMP1(-/-)) locusts. Moreover, calcium imaging in the antennal lobe of the brain revealed a substantially reduced activation of projection neurons in SNMP1(-/-) individuals upon exposure to PAN, indicating that the diminished antennal responsiveness to PAN in mutants affects pheromone-evoked neuronal activity in the brain. Furthermore, in behavioral experiments, PAN-induced effects on pairing and mate choice were altered in SNMP1(-/-) locusts. Conclusions Our findings emphasize the importance of SNMP1 for chemical communication in a hemimetabolous insect pest. Moreover, they show that SNMP1 plays a crucial role in pheromone detection that goes beyond long-chain aliphatic substances and includes aromatic compounds controlling reproductive behaviors.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序