Cross-priming amplification targeting the 18S rRNA gene for the rapid diagnosis of Babesia bovis infection

文献类型： 外文期刊

第一作者： Wang, Jinming

作者： Wang, Jinming;Gao, Shandian;Yang, Jifei;Liu, Junlong;Li, Youquan;Luo, Jianxun;Guan, Guiquan;Yin, Hong;Yin, Hong

作者机构：

关键词： Cross-priming amplification; Diagnosis; Bovine babesiosis; Babesia bovis

期刊名称：TICKS AND TICK-BORNE DISEASES （ 影响因子：3.744； 五年影响因子：3.693 ）

ISSN： 1877-959X

年卷期： 2021 年 12 卷 4 期

页码：

收录情况： SCI

摘要： Babesia bovis is a known causative agent of bovine babesiosis and is widely distributed across China. Rapid detection and accurate identification of B. bovis is essential for follow-up management and epidemiological investigations. In this study, a cross-priming amplification combined with vertical flow (CPA-VF) assay was developed. The detection limit of the CPA-VF assay targeting the 18S rRNA gene was 320 fg per reaction at 61 degrees C for 60 min. No cross-reactions were observed with other piroplasms infective to cattle. Furthermore, 36 blood samples from experimentally-infected animals were accurately assessed using the CPA-VF assay. The performance of the CPA-VF assay was compared with the results of conventional PCR for 219 blood samples from the field. Our results demonstrate that the CPA-VF assay is a practical and effective diagnostic tool for bovine babesiosis caused by B. bovis infection.

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