Molecular Characteristics of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Strains Isolated from Diseased Captive Giant Pandas (Ailuropoda melanoleuca) in China

文献类型： 外文期刊

第一作者： Ji, Xue

作者： Ji, Xue;Liu, Jun;Liang, Bing;Sun, Shiwen;Zhu, Lingwei;Guo, Xuejun;Sun, Yang;Ji, Xue;Liu, Jun;Liang, Bing;Sun, Shiwen;Zhu, Lingwei;Guo, Xuejun;Sun, Yang;Zhou, Wei;Sun, Yang

作者机构：

关键词： giant panda; Escherichia coli; antimicrobial resistance; virulence; genome sequencing

期刊名称：MICROBIAL DRUG RESISTANCE （ 影响因子：2.706； 五年影响因子：2.927 ）

ISSN： 1076-6294

年卷期： 2022 年 28 卷 6 期

页码：

收录情况： SCI

摘要： Objectives: To characterize the antimicrobial resistance and virulence of pathogenic Escherichia coli isolated from diseased captive giant pandas.Methods: Antimicrobial susceptibility and minimum inhibitory concentration (MIC) were determined by the broth dilution method. Whole-genome sequencing was used to characterize the phylogeny, serotype, virulence, resistome, plasmids, and genetic structures of the cefotaxime (CTX)-M genes.Results: Four extended-spectrum beta-lactamase (ESBL)-producing E. coli strains were identified and the MICs against 11 antibiotics in vitro were determined. All ESBL-producing E. coli strains were resistant to more than eight antibiotics and carried the bla(CTX-M-55) or bla(CTX-M-105) gene in different sizes of replicon-type plasmids (pAMSH1-IncHI2, 257 kb; pAMPD2-IncFII, 89 kb; pAMPD02-IncFIB, 129 kb; and pAMSC4-IncN, 47 kb). Distinct insertional sequences and transposases were identified up-/downstream of bla(CTX-Ms), including IS26, ISEcp1, ISKpn72, IS903B, and Tn2. These strains also possessed at least three virulence genes of pathogenic E. coli and originated in four different evolutionary branches. One strain carried the complete locus of the enterocyte effacement pathogenicity island, but lacked the virulence genes stx and bfpA, indicating atypical enteropathogenic E. coli, whereas the other strains were considered to be extraintestinal pathogenic E. coli.Conclusions: The emergence of ESBL-producing pathogenic E. coli strains from diseased captive giant pandas warrants greater attention. The findings of this study will help to prevent the spread of these strains among captive giant pandas as well as from wild animals to humans.

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