文献类型： 外文期刊
第一作者： Wu, Suting
作者： Wu, Suting;Kyaw, Htin;Yang, Yirong;Wang, Zhiwei;Zhang, Liying;Zhang, Zhiguo;Quick, William Paul;Lu, Tiegang;Cui, Xue'an;Qin, Guannan;Qin, Guannan;Deng, Lihua;Xiao, Guoying;Quick, William Paul;Tong, Zhijun;Xiao, Bingguang
作者机构：
关键词： CRISPR/Cas9; Multiplex genome editing; Assembly system; Plant
期刊名称：CROP JOURNAL （ 影响因子：6.6； 五年影响因子：6.5 ）
ISSN： 2095-5421
年卷期： 2024 年 12 卷 2 期
页码：
收录情况： SCI
摘要： The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics and genetic improvement. Currently available genome-editing tools have a limited number of targets, restricting their application in genetic research. In this study, we developed a novel CRISPR/Cas9 plant ultra-multiplex genome editing system consisting of two template vectors, eight donor vectors, four destination vectors, and one primer-design software package. By combining the advantages of Golden Gate cloning to assemble multiple repetitive fragments and Gateway recombination to assemble large fragments and by changing the structure of the amplicons used to assemble sgRNA expression cassettes, the plant ultra-multiplex genome editing system can assemble a single binary vector targeting more than 40 genomic loci. A rice knockout vector containing 49 sgRNA expression cassettes was assembled and a high co-editing efficiency was observed. This plant ultra-multiplex genome editing system advances synthetic biology and plant genetic engineering. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
分类号：
- 相关文献
作者其他论文 更多>>
-
Mechanism of hydrocolloids effect on buckwheat starch gels from interaction and structural perspectives: A comparative study
作者：Hu, Weiwei;Gu, Junchao;Zhang, Zhiguo;Wu, Weicheng;Gu, Junchao;Yang, Kai;Bu, Tingting;Natallia, Komarova
关键词：Tartary buckwheat starch; Hydrocolloid; Properties modification
-
Insights into the adsorption performance and mechanism of novel 3D bimetallic MOF nanosheets for the high-efficient removal of 6PPD and 6PPD-quinone
作者：Wu, Nannan;Wang, Jiao;Liu, Zhenzhen;Zhao, Huiyu;Di, Shanshan;Wang, Zhiwei;Wang, Xinquan;Qi, Peipei;Zheng, Bing;Zheng, Bing;Di, Shanshan;Wang, Zhiwei;Wang, Xinquan;Qi, Peipei;Di, Shanshan;Wang, Zhiwei;Wang, Xinquan;Qi, Peipei
关键词：3D bimetallic MOFs; 6PPD; 6PPD-quinone; Adsorption mechanism; DFT calculations
-
Different Efficacy of Five Soluble Dietary Fibers on Alleviating Loperamide-Induced Constipation in Mice: Influences of Different Structural Features
作者：Zhang, Zhiguo;Liu, Buyu;Zhang, Chengcheng;Hu, Weiwei;Wu, Weicheng;Liu, Buyu;Liu, Xingquan;Liu, Wei
关键词：soluble dietary fiber; structural difference; constipation; defecation function; short-chain fatty acid; gut microbiota
-
Effect of Flammulina velutipes polysaccharidees on the quality and digestibility of fresh wet rice noodles: A study perspective from physicochemical and postprandial glucose homeostasis in vivo
作者：Bu, Tingting;Kong, Xiao;Ren, Yuting;Cai, Ming;Sun, Peilong;Yang, Kai;Bu, Tingting;Kong, Xiao;Ren, Yuting;Cai, Ming;Sun, Peilong;Yang, Kai;Zhang, Zhiguo;Hu, Weiwei;Wu, Weicheng;Natallia, Komarova
关键词：Plasma hormone; Postprandial glycemic response; Rice noodles; Starch digestibility; Structure; Flammulina velutipes polysaccharides
-
Transcriptomic and metabolomic insights into the enantioselective effects of chiral fungicide penflufen and its enantiomers on multi-endpoints in the liver for mice
作者：Di, Shanshan;Bao, Zhiwei;Zhao, Huiyu;Liu, Zhenzhen;Qi, Peipei;Wang, Zhiwei;Wang, Xinquan;Di, Shanshan;Zhao, Huiyu;Liu, Zhenzhen;Qi, Peipei;Wang, Zhiwei;Wang, Xinquan;Bao, Zhiwei;Jin, Yuanxiang
关键词：Metabolomics; Transcriptomics; Chiral penflufen; Enantioselective effects; Transcriptomic analysis; Metabolomics analysis; Male mice
-
Lodging Resistance of Japonica Hybrid Rice Plants Studied in Relation to Mechanical and Physicochemical Characteristics
作者：Zhang, Liying;Ma, Zuobin;He, Na;Tang, Zhiqiang;Wang, Changhua;Zheng, Wenjing;Gao, Hong;Wang, Lili;Wang, Hui;Sui, Guomin
关键词：lodging resistance mechanisms; stem structural; chemical properties; mechanical strength evaluation; crop improvement
-
Cadmium contamination impairs alkaline tolerance in sugar beet by inhibiting carbon fixation and tryptophan metabolism
作者：Ye, Shuo;Zhou, Xinrui;Tang, Fawei;Qu, Ruixin;Wang, Zhiwei;Hu, Xu;Liu, Yang;Li, Donglin;Liu, Lei;Gai, Zhijia;Li, Xiangnan;Liu, Lei;Zhang, Pengfei;Yang, Kepan;Yang, Kepan
关键词：Alkaline; Cadmium; Carbohydrate metabolism; Combined stresses; Melatonin
