Identification of the murine osteoblastic cell MC3T3-E1 as a permissive cell line in response to lumpy skin disease virus
文献类型： 外文期刊
第一作者： You, Ting
作者： You, Ting;Wang, Xiangwei;Yin, Xiangping;Sun, Yuefeng;Chen, Hao-tai;Ren, Shanhui;You, Ting;Wang, Meng;Zhang, Hongqiang;Wang, Guirong;Gao, Xiaolong
作者机构：
关键词： First identification; Lumpy skin disease virus; Permissive cell
期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：3.1； 五年影响因子：2.4 ）
ISSN： 0166-0934
年卷期： 2024 年 326 卷
页码：
收录情况： SCI
摘要： Lumpy skin disease virus (LSDV) is a rapidly emerging pathogen in China. Screening suitable cells for LSDV replication is vital for future research on pathogenic mechanisms and vaccine development. Previous comparative studies have identified that the rodent-derived BHK21 is a highly susceptible cell model to LSDV infection. Using western blot, indirect immune-fluorescence assay, flow cytometry, and transmission electron microscopy methods, this study is the first to identify the murine osteoblastic cell line MC3T3-E1 as a novel permissive cell model for LSDV infection. The establishment of MC3T3-E1 as a suitable infectious cell model enhances our understanding of the species range and cell types of the permissive cells and nonpermissive that support LSDV replication. It is helpful to accelerate future research on the pathogenesis, clinical application, and vaccine development of LSDV.
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