Identification of Carboxylesterase Genes and Function of Three Inducible α-Esterase Genes Involved in Insecticidal Activity of α-Solanine in Phthorimaea operculella

文献类型： 外文期刊

第一作者： Ji, Yujie

作者： Ji, Yujie;Yan, Junjie;Gao, Yulin;Palli, Subba Reddy;Gao, Yulin

作者机构：

关键词： bioinformatics analysis; expression profiling; PoCarE activity; insecticidalactivity; moleculardocking

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 31 期

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收录情况： SCI

摘要： Carboxylesterases (CarEs) play an important role in the detoxification of exogenous toxic compounds in insects. However, the effect of Phthorimaea operculella CarEs (PoCarEs) on the insecticidal activity of alpha-solanine, a potato-derived secondary metabolite, remains unclear. In this study, we identified 96 PoCarEs. Phylogenetic analysis demonstrated that PoCarEs could be classified into nine clades, with an abundance of alpha-esterases. Their chromosome distribution and sequence characterization were also performed. Expression profiles of PoCarE genes revealed functional diversities in tissues and larval guts across different developmental stages. PoCarE activity increased following alpha-solanine exposure and mediated the insecticidal activity of alpha-solanine. alpha-solanine exposure increased the expression of Poae2, Poae21, and Poae25, and knockdown of these genes increased the insecticidal activity of alpha-solanine against larvae. Moreover, homology modeling and molecular docking results suggested that Poae2, Poae21, and Poae25 proteins exhibit effective affinities for alpha-solanine. These results provide valuable insights into how insects cope with alpha-solanine toxicity, facilitating the development of an efficient management strategy for this and other pests.

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