Genetic engineering and raising temperature enhance recombinant protein production with the cdna1 promoter in Trichoderma reesei

文献类型： 外文期刊

第一作者： Jiang, Shanshan

作者： Jiang, Shanshan;Wang, Yue;Liu, Qin;Zhao, Qinqin;Song, Xin;Li, Xuezhi;Qu, Yinbo;Liu, Guodong;Gao, Liwei

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关键词： Trichoderma reesei; Promoter engineering; Protein expression; Heat induction; beta-mannanase

期刊名称：BIORESOURCES AND BIOPROCESSING （ 影响因子：4.983； ）

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年卷期： 2022 年 9 卷 1 期

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收录情况： SCI

摘要： The fungus Trichoderma reesei is a powerful host for secreted production of proteins. The promoter of cdna1 gene, which encodes a small basic protein of unknown function and high expression, is commonly used for constitutive protein production in T. reesei. Nevertheless, the production level of proteins driven by this promoter still needs to be improved. Here, we identified that the region 600- to 700-bp upstream of the start codon is critical for the efficiency of the cdna1 promoter. Increasing the copy number of this region to three improved the production of a heterologous beta-mannanase by 37.5%. Screening of several stressful conditions revealed that the cdna1 promoter is heat inducible. Cultivation at 37 degrees C significantly enhanced the production of beta-mannanase as well as a polygalacturonase with the cdna1 promoter compared with those at 30 degrees C. Combing the strategies of promoter engineering, multi-copy gene insertion, and control of cultivation temperature, beta-mannanase of 199.85 U/mL and relatively high purity was produced in shake flask, which was 6.6 times higher than that before optimization. Taken together, the results advance the understanding of the widely used cdna1 promoter and provide effective strategies for enhancing the production of recombinant proteins in T. reesei.

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