Resistance to both aphids and nematodes in tobacco plants expressing a Bacillus thuringiensis crystal protein

文献类型： 外文期刊

第一作者： Wang, Yong

作者： Wang, Yong;Wang, MengNan;Zhang, Yali;Li, Shengchun;Zhang, Jiang;Zhang, Fengjuan;Chen, Feng;Sun, Ming;Zhang, Jiang;Zhang, Jiang;Zhang, Fengjuan

作者机构：

关键词： Bacillus thuringiensis Cry5Ba2 protein; pest control; Myzus persicae; Meloidogyne incognita

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： BACKGROUND: Bacillus thuringiensis (Bt) and its crystal toxin or delta-endotoxins (Cry) offer great potential for the efficient control of crop pests. A vast number of pests can potentially infect the same host plant, either simultaneously or sequentially. However, no effective Bt-Cry protein has been reported to control both aphids and plant parasitic nematodes due to its highly specific activity. RESULTS: Our study indicated that the Cry5Ba2 protein was toxic to the green peach aphid Myzus persicae, which had a median lethal concentration (LC50) of 9.7 ng mu L-1 and fiducial limits of 3.1-34.6 ng mu L-1. Immunohistochemical localization of Cry5Ba2 revealed that it could bind to the apical tip of microvilli in midgut regions. Moreover, transgenic tobacco plants expressing Cry5Ba2 exhibited significant resistance to Myzus persicae, as evidenced by reduced insect survival and impaired fecundity, and also intoxicated the Meloidogyne incognita as indicated by a decrease in galls and progeny reproduction. CONCLUSION: In sum, we identified a new aphicidal Bt toxin resource that could simultaneously control both aboveground and belowground pests, thus extending the application range of Bt-based strategy for crop protection. (c) 2024 Society of Chemical Industry.

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