Identification and characterization of biocontrol agent Lysinibacillus boronitolerans P42 against Cerrena unicolor that causes root rot of arecanut palm

文献类型： 外文期刊

第一作者： Ma, Xiang

作者： Ma, Xiang;Gao, Yuxiao;Li, Hong;Li, Juanjuan;Tang, Yanqiong;Liu, Zhu;Wang, Dan;Hu, Xinwen;Huang, Xi;Lin, Min

作者机构： Hainan Univ, Sch Life Sci, Minist Educ, Key Lab Trop Biol Resources, Haikou 570228, Peoples R China;Hainan Univ, Sch Trop Crops, Haikou 570228, Peoples R China;Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词： Lysinibacillus boronitolerans; Biocontrol; Antagonistic activity; Fermentation filtrate

期刊名称：ARCHIVES OF MICROBIOLOGY （ 2022影响因子：2.8； 五年影响因子：2.8 ）

ISSN： 0302-8933

年卷期： 2023 年 205 卷 4 期

页码：

收录情况： SCI

摘要： The arecanut palm is one of the most important industrial crops in tropical area around the world. The root rot of arecanut palm, which is caused by Cerrena unicolor, has led to heavy economic losses and restricted greatly the development of arecanut industry, especially in Hainan province of China. The common use of chemical agents has worsened the problems of the emergence of resistant pathogens and the pollution of agricultural environment. This study aims to screen and identify a more effective and environment friendly biocontrol method for the prevention and treatment of root rot of arecanut palm. The mycelium growth rate is investigated to select antagonistic bacteria from tropical crop rotation fields which show improved resistance against soil-borne pathogens, and the strain P42 is revealed with the strongest antagonistic effects (82.18%). Based on 16 s rDNA sequence analysis, the strain P42 is identified as Lysinibacillus boronitolerans. In vitro antimicrobial activity shows that the strain P42 exhibits broad-spectrum antagonistic activity against a wide variety of tropical agricultural fungal pathogens, including Cerrena unicolor, Magnaporthe oryzea, Botryodiplodia theobromae, Neoscytalidium dimidiatum, Thanatephorus cucumeris, Fusarium oxysporum, and Botrytis cinerea Per.. The antagonistic activity of the culture of P42 is tolerant to common proteases, longer storage time, and temperature range of 40-121 degrees C; and is significantly influenced by alkaline (7-9) and acidic (1-2) pH, as well as by ultraviolet ray treatment for more than 30 min. The investigation on the antagonistic activity of the crude extract of fermentation filtrate indicates that the active compounds might be lipopeptides, polyketones, or proteins. To our knowledge, this is the first report of L. boronitolerans as potential bio-reagents for controlling root rot of arecanut palm caused by Cerrena unicolor.

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