Milk-derived antimicrobial peptide GMp7: Disrupting protein networks for multi-target antibacterial inhibition and enhanced dairy preservation

文献类型： 外文期刊

第一作者： He, Jinze

作者： He, Jinze;Xu, Yujuan;Yu, Yuanshan;He, Jinze;Li, Hong;Li, Yufang;Yang, Tingting;Yu, Xiaoyan;Yang, Xue;Huang, Aixiang;Shi, Yanan;Li, Hong

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关键词： milk-derived peptide; protein-protein; network; central proteins; preservation

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0022-0302

年卷期： 2025 年 108 卷 4 期

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收录情况： SCI

摘要： Our goal was to identify and characterize a novel milk-derived antimicrobial peptide, GMp7, and reveal antibacterial mechanism against Staphylococcus aureus. Therefore, this study revealed the multi-target antibacterial mechanism of GMp7 on S. aureus DC.RB-015 by Fourier-transform infrared spectroscopy, flow cytometry, scanning electron microscopy, and label-free proteomics analysis. The results showed that GMp7 has a secondary structure consisting of 17.45% alpha-helix, 20.10% (3-corner, and 37.13% (3-fold, which is conducive to membrane disruption and bacterial cell death. Treatment with GMp7 induced a significant change in the proteome, resulting the downregulation of 99 proteins and the upregulation of 26 proteins, indicating a multi-target effect on bacterial physiology. Notably, GMp7 forms stable bonds with the critical proteins asd and pcrA within the S. aureus protein network, which includes a larger network of proteins essential for bacterial viability. The binding energies for GMp7 with asd and pcrA were determined be -8.477 and -8.407 kcal/mol, respectively. GMp7 exerts its bacteriostatic effects through multiple pathways, including interference with peptidoglycan biosynthesis, ATP-binding cassette transport, the 2-component system, DNA replication, and mismatch repair. The efficacy the peptide against S. aureus in pasteurized milk suggests its potential use in the dairy industry to improve product shelf life and safety. In addition, GMp7 significantly inhibited the growth of S. aureus in milk. These findings have practical significance for the use of antimicrobial peptides to control bacterial contamination in food and improve food safety.

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