Intestinal fungi biogeography, succession and its association with diarrhea in pigs

文献类型： 外文期刊

第一作者： Ren, Ruochen

作者： Ren, Ruochen;Ma, Lingyan;Xiao, Yingping;Chen, Qu;Wen, Yang;Lyu, Wentao;Zhang, Xiaojun;Lou, Fangfang;Li, Yang

作者机构：

关键词： Diarrhea; Fungi; Growth stage; Mycobiome; Pigs

期刊名称：JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY （ 影响因子：6.5； 五年影响因子：7.2 ）

ISSN： 1674-9782

年卷期： 2025 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Background The composition and relative abundances of intestinal microbiota are closely related to animal growth, development and health. This study provides a comprehensive analysis of the spatial distribution and temporal dynamics of intestinal fungi in pigs, with a focus on fungal alterations associated with diarrhea. Methods Intestinal digesta from duodenum, jejunum, ileum, cecum, colon and feces of 8 finishing pigs (180 days old) were collected. Fecal samples were also collected from 18 pigs across different growth stages, including lactation (3 d), nursery (26 d, 35 d, 49 d), growing (120 d) and finishing (180 d). Additionally, feces were collected from 32 diarrheal and 32 healthy piglets at 28 days old. Fungal community profiling in these samples was performed using internal transcribed spacer (ITS) sequencing. Results A total of 9,224 amplicon sequence variants (ASVs) were detected in all of 220 samples. Intestinal fungal diversity exhibited clear biogeographic patterns, with significantly lower richness and Shannon index in the ileum (P < 0.05) and significantly higher richness in the large intestine and feces (P < 0.05). The fungal community structure also varied significantly across intestinal segments, with Kazachstania dominating in the ileum and Geotrichum in the duodenum and jejunum. Across growth stages, fecal fungal diversity increased after weaning. PCoA results revealed that fungal structure exhibited significant temporal changes (R = 0.7313, P = 0.001), with the core fungal taxa dominated by Diutina catenulata, Aspergillus restrictus and Tahromyces munnarensis. In addition, by comparing the fungal community of piglets with and without diarrhea, the richness and Shannon index were significantly higher in the diarrheal piglets than those in healthy piglets (P < 0.05) with Kazachstania, Diutina and Aspergillus enriched in diarrheal piglets and Geotrichum, Tahromyces and Piromyces in healthy piglets. Conclusions The intestinal fungal community in pigs shows distinct spatial variation, with greater diversity in the large intestine. Fungal composition shifts dynamically with age, particularly around the weaning transition. This study highlights specific fungal taxa associated with diarrhea caused by weaning stress, offering new insights into the interplay between gut fungi and pig health.

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