Improving plant C-to-G base editors with cold-adapted glycosylase and TadA-8e variants

文献类型： 外文期刊

第一作者： Jiang, Yingli

作者： Jiang, Yingli;Xiao, Zhi;Zhou, Suhuai;Tong, Chaoyun;Jin, Shan;Liu, Xiaoshuang;Wei, Pengcheng;Xiao, Zhi;Tong, Chaoyun;Jin, Shan;Qin, Ruiying;Xu, Rongfang;Li, Juan;Xiao, Zhi;Zhou, Suhuai;Wei, Pengcheng;Luo, Zhaopeng;Pan, Lang

作者机构：

期刊名称：TRENDS IN BIOTECHNOLOGY （ 影响因子：14.9； 五年影响因子：16.6 ）

ISSN： 0167-7799

年卷期： 2025 年 43 卷 7 期

页码：

收录情况： SCI

摘要： Plant cytosine (C)-to-guanine (G) base editors (CGBEs) have been established but suffer from limited editing efficiencies and low outcome purities. This study engineered a cod uracil DNA glycosylase (cod UNG, coUNG) from the cold-adapted fish Gadus morhua for plant CGBE, demonstrating 1.71-to 2.54-fold in-creases in C-to-G editing efficiency compared with the CGBE using human UNG (HUNG). Further engineering took advantage of TadA-8e-derived cytidine deaminases (TadA-CDs). These variants induced C substitutions with efficiencies ranging from 26.28% to 30.82% in rice cells, whereas adenine (A) conversion was negligible. By integrating coUNG and TadA-CDc elements with SpCas9 nickase, the resulting CDC-CGBEco achieved pure C-to-G editing without byproducts in up to 52.08% of transgenic lines. Whole-genome sequencing (WGS) analysis revealed no significant off-target effects of the CDC-BEs in rice. In addition, CDC-CGBEco enabled precise C-to-G editing in soybean and to-bacco. These engineered CGBEs enhanced editing efficiency, purity, and specificity, suggesting their broad potential for applications in scientific research and crop breeding.

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