PI3K/AKT/mTOR, NF-Kappa B and ERS pathway participated in the attenuation of H2O2-induced IPEC-J2 cell injury by koumine

文献类型： 外文期刊

第一作者： Yuan, Zhihang

作者： Yuan, Zhihang;Yang, Mengran;Yang, Chenglin;Kong, Xiangyi;Wu, You;Wang, Siqi;Fan, Hui;Ning, Can;Xiao, Wenguang;Sun, Zhiliang;Wu, Jing;Yuan, Zhihang;Yang, Mengran;Yang, Chenglin;Kong, Xiangyi;Wu, You;Wang, Siqi;Fan, Hui;Ning, Can;Xiao, Wenguang;Sun, Zhiliang;Wu, Jing;Sun, Zhiliang;Liang, Zengenni;Sun, Zhiliang

作者机构：

关键词： Koumine; IPEC-J2; Inflammation; ERS; ROS; Apoptosis; Autography; NF-?B; PI3K

期刊名称：JOURNAL OF ETHNOPHARMACOLOGY （ 影响因子：5.4； 五年影响因子：5.3 ）

ISSN： 0378-8741

年卷期： 2023 年 304 卷

页码：

收录情况： SCI

摘要： Ethnopharmacological relevance: Koumine, an indole alkaloid extracted from Gelsemium elegans Benth, exerts anti-inflammation and antioxidant activities. However, the effects of koumine on intestinal injury induced by H2O2 and its potential molecular mechanisms need larger studies. Aim of the study: We established an IPEC-J2 cell damage model induced by H2O2 to explore the protective mechanism of koumine on intestinal injury.Materials and methods: In the experiment, cell damage models were made with hydrogen peroxide. To assess the protective effect of koumine on H2O2-induced IPEC-J2 cell injury, CCK-8, the release of LDH and ROS, trans-mission electron microscopy and Annexin V-FITC/PI were employed. Western Blot and Quantitative Real-time PCR were used to determine the potential alleviated mechanism of koumine on H2O2-trigged IPEC-J2 cell damage.Results: The results of CCK-8 and LDH implied that koumine has a mitigative effect on H2O2-induced cell damage via upregulating cell viability and suppressing cell membrane fragmentation. Simultaneously, koumine notably inhibited the level of pro-inflammatory factors (IL-1 beta, IL-6, IL-8, TNF-alpha and TGF-beta), the over-production of ROS along with decreasing the injury of mitochondrion, endoplasmic reticulum and lysosome induced by H2O2. Moreover, koumine dramatically attenuated H2O2-triggered IPEC-J2 cell apoptosis and autophagy. Subse-quently, Western blot analysis identified NF-Kappa B, PI3K and ERS as possible pathway responsible for the protective effect of koumine on H2O2-stimulated IPEC-J2 cell inflammation.

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