Genetic Variation in ZmPAT7 Contributes to Tassel Branch Number in Maize

文献类型： 外文期刊

第一作者： Guan, Honghui

作者： Guan, Honghui;Chen, Xiaojing;Wang, Kailiang;Liu, Xuyang;Zhang, Dengfeng;Li, Yongxiang;Song, Yanchun;Shi, Yunsu;Wang, Tianyu;Li, Chunhui;Li, Yu

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关键词： maize (Zea mays L; ); tassel branch number (TBN); ZmPAT7; protein S-acyltransferases (PAT); expression quantitative trait locus (eQTL)

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

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年卷期： 2022 年 23 卷 5 期

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收录情况： SCI

摘要： Tassel branch number (TBN) is one of the important agronomic traits that contribute to the efficiency of seed production and has been selected strongly during the modern maize breeding process. However, the genetic mechanisms of TBN in maize are not entirely clear. In this study, we used a B73 x CML247 recombination inbred lines (RILs) population to detect quantitative trait loci (QTLs) for TBN. A total of four QTLs (qTBN2a, qTBN2b, qTBN4, and qTBN6) and six candidate genes were identified through expression analysis. Further, one of the candidates (GRMZM2G010011, ZmPAT7) encoding an S-acyltransferase was selected to validate its function by CRISPR-Cas9 technology, and its loss-of-function lines showed a significant increase in TBN. A key SNP(-101) variation in the promoter of ZmPAT7 was significantly associated with TBN. A total of 17 distant eQTLs associated with the expression of ZmPAT7 were identified in expression quantitative trait loci (eQTL) analysis, and ZmNAC3 may be a major factor involved in regulating ZmPAT7. These findings of the present study promote our understanding of the genetic basis of tassel architecture and provide new gene resources for maize breeding improvement.

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