Molecular and expression analysis of apolipoprotein E gene in the Chinese sturgeon, Acipenser sinensis

文献类型： 外文期刊

第一作者： Li, Chuang-Ju

作者： Li, Chuang-Ju;Chen, Xi-Hua;Liu, Zhi-Gang;Wei, Qi-Wei;Tang, Yong-Kai;Li, Chuang-Ju;Gan, Fang;Chen, Xi-Hua;Liu, Zhi-Gang;Li, Luo-Xin;Wei, Qi-Wei

作者机构：

关键词： Apolipoprotein E;Chinese sturgeon;Cloning;RT-PCR;gene expression

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

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收录情况： SCI

摘要： Apolipoproteins are carrier proteins that bind to lipids to form lipoprotein particles and have been shown to play an important role in lipid metabolism. In this study, a full-length cDNA for apolipoprotein E, named AsapoE, was cloned from the Chinese sturgeon (Acipenser sinensis). This cDNA sequence is 1289. bp in length, and codes for a polypeptide of 274 amino acid residues, which is 45% and 42% identical to that of the rainbow trout and zebrafish, respectively, and 39%, 30%, and 29% identical to frog, mouse, and human respectively. The predicted AsApoE protein has a conserved amphipathic α-helix region with the potential to bind to lipids. RT-PCR analysis reveals that AsapoE is expressed in all tissues examined with a preferential expression in the kidney and liver. During the embryo development stage, AsapoE mRNA is low but still detectable at gastrula stage embryos; then AsapoE mRNAs reach a higher level in muscle contraction stage embryos, this relatively stable expression persists during the following embryogenic stages and declines 1. day after hatching. These results will serve as a basis for comparative studies on vertebrate apoE genes.

分类号： Q5

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