Identification of three Superoxide dismutase genes from a Geobacillus sp.

文献类型： 外文期刊

第一作者： Zou, Yuanyuan

作者： Zou, Yuanyuan;Yang, Ling;Liu, Guoxian;Li, Yinv;Zhang, Zhifang;Zou, Yuanyuan;Zhu, Yuexiong

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期刊名称：PROTEIN JOURNAL （ 影响因子：2.371； 五年影响因子：1.929 ）

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收录情况： SCI

摘要： We report the characterization of three Superoxide dismutase (sod) genes isolated from a bacterium in the Geobacillus genus. We isolated the bacterium from high-temperature pond mud and used 16S rRNA gene sequence to confirm its identity in the Geobacillus genus. The three genes Mn-sod, Fe/Mn-sod, and Cu/Zn-sod were cloned and analyzed. Their open reading frames are Mn-sod: 615 bp encoding a 204 amino acid protein; Fe/Mn-sod: 1,236 bp encoding a 411 amino acid protein; Cu/Zn-sod: 522 bp encoding a 173 amino acid protein. When these sod genes were expressed in Escherichia coli, only Mn-SOD was able to be purified. The activity of the purified Mn-SOD we got was about 2,730 U/mg. Studies of this Mn-SOD showed that it was thermostable at 60 degrees , had 70% activity at 80 degrees after 2.5 h, and still had 30% activity at 90 degrees after 2.5 h. Mn-SOD activity required the ion Mn(2)+. Based on gel electrophoresis, we deduced that this Mn-SOD was a homotetramer. No activity was detected after the other two genes (Fe/Mn-sod, Cu/Zn-sod) were expressed in Escherichia coli, but activities were detected when expressed in Pichia pastoris.

分类号： Q51

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