文献类型： 外文期刊
第一作者： Zou, Yuanyuan
作者： Zou, Yuanyuan;Yang, Ling;Liu, Guoxian;Li, Yinv;Zhang, Zhifang;Zou, Yuanyuan;Zhu, Yuexiong
作者机构：
期刊名称：PROTEIN JOURNAL （ 影响因子：2.371； 五年影响因子：1.929 ）
ISSN：
年卷期：
页码：
收录情况： SCI
摘要： We report the characterization of three Superoxide dismutase (sod) genes isolated from a bacterium in the Geobacillus genus. We isolated the bacterium from high-temperature pond mud and used 16S rRNA gene sequence to confirm its identity in the Geobacillus genus. The three genes Mn-sod, Fe/Mn-sod, and Cu/Zn-sod were cloned and analyzed. Their open reading frames are Mn-sod: 615 bp encoding a 204 amino acid protein; Fe/Mn-sod: 1,236 bp encoding a 411 amino acid protein; Cu/Zn-sod: 522 bp encoding a 173 amino acid protein. When these sod genes were expressed in Escherichia coli, only Mn-SOD was able to be purified. The activity of the purified Mn-SOD we got was about 2,730 U/mg. Studies of this Mn-SOD showed that it was thermostable at 60 degrees , had 70% activity at 80 degrees after 2.5 h, and still had 30% activity at 90 degrees after 2.5 h. Mn-SOD activity required the ion Mn(2)+. Based on gel electrophoresis, we deduced that this Mn-SOD was a homotetramer. No activity was detected after the other two genes (Fe/Mn-sod, Cu/Zn-sod) were expressed in Escherichia coli, but activities were detected when expressed in Pichia pastoris.
分类号： Q51
- 相关文献
作者其他论文 更多>>
-
Screening of Active Substances Regulating Alzheimer's Disease in Ginger and Visualization of the Effectiveness on 6-Gingerol Pathway Targets
作者：Pan, Yecan;Li, Zishu;Zhao, Xiaoyu;Du, Yang;Zhang, Lin;Lu, Yushun;Yang, Ling;Cao, Yilin;Qiu, Jing;Qian, Yongzhong;Pan, Yecan;Li, Zishu;Zhao, Xiaoyu;Du, Yang;Zhang, Lin;Lu, Yushun;Yang, Ling;Cao, Yilin;Qiu, Jing;Qian, Yongzhong
关键词：6-Gingerol; ginger; Alzheimer's disease (AD); network pharmacology; molecular dynamics (MD)
-
A SARS-CoV-2 Nanobody Displayed on the Surface of Human Ferritin with High Neutralization Activity
作者：Zhang, Wenrong;Wang, Haining;Wu, Tong;Gao, Xintao;Shang, Yuting;Zhang, Zhifang;Liu, Xingjian;Li, Yinu;Zhang, Wenrong
关键词：COVID-19; ferritin; fenobody; baculovirus expression system; silkworm
-
Effects of bisphenol A and tauroursodeoxycholic acid on maturation of porcine oocytes and parthenogenetic development of embryos
作者：Yang, Ling;Zhang, Qiongao;Zhang, Leying;Zhang, Qiongao;Cui, Maosheng;Ma, Yong
关键词：bisphenol A; embryo; oocyte; pig; tauroursodeoxycholic acid
-
Puerariae Radix protects against ulcerative colitis in mice by inhibiting NLRP3 inflammasome activation
作者：Ga, Yu;Wei, Yuanyuan;Zhao, Qingyu;Fan, Yimeng;Zhang, Yannan;Hao, Zhihui;Ga, Yu;Wei, Yuanyuan;Zhao, Qingyu;Fan, Yimeng;Zhang, Yannan;Hao, Zhihui;Zhang, Zhifang;Hao, Sijia;Wang, Lixia;Wang, Zhifen;Han, Jinlong;Wu, Shuang
关键词：Ulcerative colitis; Molecular mechanisms; Pyroptosis
-
Genome characteristics and the ODV proteome of a second distinct alphabaculovirus from Spodoptera litura
作者：Gao, Weisong;Liu, Xingjian;Gao, Xintao;Wu, Tong;Wei, Shuang;Zhang, Zhifang;Li, Yinue;Zhang, Huan
关键词：Spodoptera litura Nucleopolyhedrovirus; Baculovirus; Genome sequence; Virus species demarcation criteria; ODV proteome
-
Ultrasonic-Assisted Extraction and Gastrointestinal Digestion Characteristics of Polysaccharides Extracted from Mallotus oblongfolius
作者：Tan, Gansheng;Duan, Zhouwei;Xin, Tian;Yang, Ling;Liu, Feng;Xie, Hui;Tan, Gansheng;Xia, Guanghua;Xin, Tian;Duan, Zhouwei;Yang, Ling;Liu, Feng;Xie, Hui
关键词：Mallotus oblongfolius polysaccharides (MOPS); ultrasonic extraction; gastrointestinal digestion; antioxidant properties; hypoglycaemia
-
Feature Selection for Explaining Yellowfin Tuna Catch per Unit Effort Using Least Absolute Shrinkage and Selection Operator Regression
作者：Yang, Ling;Zhou, Weifeng;Yang, Ling
关键词：CPUE; Lasso; feature selection; machine learning; fisheries development; yellowfin tuna
