Quantitative measurement of pH influence on SalR regulated gene expression in Acinetobacter baylyi ADP1
文献类型: 外文期刊
第一作者: Li, Chao
作者: Li, Chao;Zhou, Lin;Zhang, Yongjun;Song, Fuping;Zhang, Jie
作者机构:
关键词: transcriptional regulation;exponential growth;pH influence;environment change
期刊名称:JOURNAL OF MICROBIOLOGICAL METHODS ( 影响因子:2.363; 五年影响因子:2.24 )
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收录情况: SCI
摘要: The influence of medium pH conditions on regulation of the sal operon was quantitatively measured by using a genetically engineered Acinetobacter baylyi ADP1 strain ADPWH_lux. Live-cell based bioluminescence assays determined that the SalR, a LysR-type transcriptional regulator (LTTR), functioned in a range of medium pH conditions. During exponential growth, SalR regulated bioluminescence production peaked at the neutral pH and tolerated medium pH changes of 5.5-9.0. In the stationary phase, the SalR regulated gene expression reached 20-35% of the maximum productivity during the exponential phase. This report reveals the influence of pH conditions on a LTTR system and demonstrates that the biosensor strain can be a useful tool for understanding transcriptional regulations in different environments. Crown Copyright
分类号: Q93-3
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