An h-Type Thioredoxin Functions in Tobacco Defense Responses to Two Species of Viruses and an Abiotic Oxidative Stress

文献类型： 外文期刊

第一作者： Sun, Lijun

作者： Sun, Lijun;Ren, Haiying;Liu, Ruoxue;Li, Baoyan;Wu, Tingquan;Sun, Feng;Liu, Huimin;Wang, Xiaomeng;Dong, Hansong;Sun, Lijun;Ren, Haiying

作者机构：

期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：4.171； 五年影响因子：4.836 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Various thioredoxin (Trx) proteins have been identified in plants. However, many of the physiological roles played by these proteins remain to be elucidated. We cloned a TRXh-like gene predicted to encode an h-type Trx in tobacco (Nicotiana tabacum) and designated it NtTRXh3, based on the biochemical activity of the NtTRXh3 protein. Overexpression of NtTRXh3 conferred resistance to Tobacco mosaic virus and Cucumber mosaic virus, both of which showed reduced multiplication and pathogenicity in NtTRXh3-overexpressing plants compared with controls. NtTRXh3 overexpression also enhanced tobacco resistance to oxidative stress induced by paraquat, an herbicide that inhibits the production of reducing equivalents by chloroplasts. The NtTRXh3 protein localized exclusively to chloroplasts in coordination with the maintenance of cellular reducing conditions, which accompanied an elevation in the glutathione/glutathione disulfide couple ratio. NtTRXh3 gene expression and NtTRXh3 protein production were necessary for these defensive responses, because they were all arrested when NtTRXh3 was silenced and the production of NtTRXh3 protein was abrogated. These results suggest that NtTRXh3 is involved in the resistance of tobacco to virus infection and abiotic oxidative stress.

分类号： Q94

  • 相关文献
作者其他论文 更多>>

微信小程序