DEVELOPMENT AND CHARACTERIZATION OF 21 EST-DERIVED MICROSATELLITE MARKERS IN VICIA FABA (FAVA BEAN)
文献类型: 外文期刊
第一作者: Ma, Yu
作者: Ma, Yu;Yang, Tao;Guan, Jianping;Wang, Shumin;Wang, Haifei;Sun, Xuelian;Zong, Xuxiao
作者机构:
关键词: allele;expressed sequence tag;simple sequence repeat markers;primer pairs;genotype;heterozygosity;GenBank sequence data;genetic diversity;polymorphism;NCBI database;EMBL database;molecular breeding;GRAMENE website;SSRIT software
期刊名称:AMERICAN JOURNAL OF BOTANY ( 影响因子:3.844; 五年影响因子:3.657 )
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年卷期:
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收录情况: SCI
摘要: Premise of the study: Expressed sequence tag (EST)-derived microsatellite simple sequence repeat (SSR) markers were developed in Vicia faba L. by screening the NCBI database. Markers were validated and explored for size polymorphism among fava bean accessions.Methods and Results: Twenty-one EST-SSR primer pairs were identified, and the loci characterized were size polymorphic among 32 fava bean genotypes from diverse geographical locations. The number of alleles (N-alpha) per locus ranged from 2 to 9, and expected heterozygosity (H-E) ranged from 0.0476 to 0.8304, respectively.Conclusions: These markers will be useful to study genetic diversity, genetic mapping, and molecular breeding in fava bean.
分类号: Q94
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