A novel strategy to improve the bioconversion of l-carnitine from crotonobetaine

文献类型： 外文期刊

第一作者： Tian, Jinqiang

作者： Tian, Jinqiang;Wang, Qiang;Zhang, Zhongyuan;Tian, Jinqiang;Wang, Qiang

作者机构：

关键词： lipase: 9001-62-1;EC 3.1.1.3;palmitoyl-L-carnitine: 2364-67-2;crotonobetaine: 927-89-9;tert-amyl alcohol: 75-85-4;L-carnitine: 541-15-1;bioconversion;carnitine dehydratase: 104382-17-4;EC 4.2.1.89

期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：2.998； 五年影响因子：3.005 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Using [Bmim]PF6 ionic liquid as reaction medium, l-carnitine was biosynthesized through hydration of crotonobetaine under the action of carnitine dehydratase. l-Carnitine was transformed into palmitoyl-l-carnitine by lipase, which was extracted with tert-amyl alcohol. With the removal of l-carnitine, biotransformation of crotonobetaine into l-carnitine was continued in the reversible hydration reaction. According to this study, the molar yield of total l-carnitine could attain 87.2% after the five-cycle intermittent extraction, which was higher than all previous reports.

分类号： R15

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