Trypanosoma (Herpetosoma) grosi: First isolation from Chinese striped field mouse (Apodemus agrarius)

文献类型： 外文期刊

第一作者： Guan, Guiquan

作者： Guan, Guiquan;Niu, Qingli;Yang, Jifei;Li, Youquan;Gao, Jinliang;Luo, Jianxun;Yin, Hong

作者机构：

关键词： genomic DNA;fetal bovine serum;Cha1

期刊名称：PARASITOLOGY INTERNATIONAL （ 影响因子：2.23； 五年影响因子：2.281 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A "lewisi-like" Trypanosoma parasite was isolated from the blood of Chinese striped field mice (Apodemus agrarius) trapped in the fields in the Garman Tibet area, Gansu province, China. The parasite was successfully cultivated in vitro in HL-1 medium supplemented 20% fetal bovine serum (FBS). Full formed spheromastigote, metacyclic trypomastigote and trypomastigote structures were all visible in films made from the culture. A nucleotide fragment of 2159-bp length was amplified from genomic DNA of the parasite using specific primers for the 18S rRNA gene of trypanosomes. The alignment indicated that this parasite had higher identities with T. (Herpetosoma) grosi (more than 99.6%) than other Herpetosoma species (less than 98.5%), which suggest that the parasite should be classified as T. (Herpetosoma) grosi. This is the first time in China that an isolation of T. (Herpetosoma) grosi is reported although several strains of T. (Herpetosoma) lewisi have been isolated from rodents of family Muridae in various provinces. Thus, it was designated as T. (Herpetosoma) grosi Chat and deposited in the center of parasite strain collection and preservation in our laboratory for future study. In addition, this culture method will be used to isolate, maintain and study the long-term development of this parasite in vitro

分类号： Q18

  • 相关文献

[1]A novel approach obtaining intron-containing hairpin RNA constructs. Cai, Ping-Zhong,Wen, Chun-Miao,Xu, Zheng-Jun,Zhong, Wan-Fang.

[2]Phenotypic and genotypic changes in rapeseed after 18 years of storage and regeneration. Wu, XM,Wu, NF,Qian, XZ,Li, RG,Huang, FH,Zhu, L. 1998

[3]Analysis of codon usage bias of mitochondrial genome in Bombyx mori and its relation to evolution. Wei, Lei,Jia, Xian,Qi, Qi,Liang, Zhisheng,Zheng, Hao,Ping, Yao,Sun, Jingchen,He, Jian,Liu, Shuyu. 2014

[4]Fine genetic mapping localizes cucumber scab resistance gene Ccu into an R gene cluster. Kang, Houxiang,Yang, Yuhong,Zhang, Zhonghua,Zhang, Shengping,Mao, Zhenchuan,Cheng, Guohua,Gu, Xingfang,Huang, Sanwen,Xie, Bingyan,Weng, Yiqun,Kang, Houxiang,Yang, Yuhong,Zhang, Zhonghua,Zhang, Shengping,Mao, Zhenchuan,Cheng, Guohua,Gu, Xingfang,Huang, Sanwen,Xie, Bingyan.

[5]Cloning and SNP screening of the TLR4 gene and the association between its polymorphism and somatic cell score in dairy cattle. Xu, Shang-zhong,Gao, Xue,Li, Jun-ya,Ren, Hong-yan,Wang, Xing-ping,Luoren, Zhuo-ma.

[6]Development and characterization of 19 microsatellite markers for Collichthys lucidus. Ma, Chunyan,Ma, Hongyu,Ma, Lingbo,Cui, Haiyu,Ma, Qunqun,Cui, Haiyu,Ma, Qunqun.

[7]Functional analysis of GUS expression patterns and T-DNA integration characteristics in rice enhancer trap lines. Peng, H,Huang, HM,Yang, YZ,Zhai, Y,Wu, JX,Huang, DF,Lu, TG.

[8]Widespread 5-methylcytosine in the genomes of avian Coccidia and other apicomplexan parasites detected by an ELISA-based method. Gong, Zhenxing,Yin, Hao,Ma, Xueting,Liu, Baohong,Han, Zhenglan,Gou, Lingqiao,Cai, Jianping,Gong, Zhenxing,Yin, Hao,Ma, Xueting,Liu, Baohong,Han, Zhenglan,Gou, Lingqiao,Cai, Jianping,Gong, Zhenxing,Yin, Hao,Ma, Xueting,Liu, Baohong,Han, Zhenglan,Gou, Lingqiao,Cai, Jianping.

[9]Construction and Characterization of a Bacterial Artificial Chromosome Library from the Huoyan Goose. Hu, Peng Fei,Li, Xiang Chen,Chen, Xian Wei,Guan, Wei Jun,Ma, Yue Hui. 2012

[10]A Simple Sequence Repeat Marker Linked to the Susceptibility of Apple to Alternaria Blotch Caused by Alternaria alternata Apple Pathotype. Zhang, Liyi,Cong, Peihua,Li, Ying,Zhang, Zhen,Cheng, Zong-Ming,Cheng, Zong-Ming.

[11]Development of SSR molecular markers for Allium mongolicum. Hu, Jing,Hu, Xiaoke,Zhang, Jinhu,Fan, Baoli,Yu, Qiushi,Zhang, Qian.

[12]Molecular cloning and characterization of GhAPm, a gene encoding the mu subunit of the clathrin-associated adaptor protein complex that is associated with cotton (Gossypium hirsutum) fiber development. Zhou, Tao,Zhang, Rui,Yang, Dawei,Guo, Sandui.

[13]Response of the panicles exserted from the caulis and from various effective tillers at four stages of panicle development to neck blast in rice. Hao, Zhongna,Wang, Lianping,Liang, Jiangen,Tao, Rongxiang.

[14]The technology of large-scale pharmaceutical plasmid purification by cetyltrimethylammonium bromide and Tritonx-114. Hou, S. H.,Zhu, H. F.,Zhang, Q.,Yuan, W. F.,Sun, H. C.. 2010

[15]High-efficiency and stable genetic transformation of pear (Pyrus communis L.) leaf segments and regeneration of transgenic plants. Sun, Qingrong,Zhao, Yan,Sun, Hongyan,Hammond, Rose W.,Davis, Robert E.,Sun, Qingrong,Sun, Hongyan,Xin, Li.

[16]Karyotype analysis of Oreochromis mossambicus, O. urolepis hornorum and their hybrid based on Cot-1 DNA bands by fluorescence in situ hybridization. Zhu, Hua Ping,Lu, Mai Xin,Huang, Zhang Han,Gao, Feng Ying,Ma, Dong Mei,Zhou, Li,Gui, Jian Fang.

作者其他论文 更多>>

微信小程序