Trypanosoma (Herpetosoma) grosi: First isolation from Chinese striped field mouse (Apodemus agrarius)

文献类型： 外文期刊

第一作者： Guan, Guiquan

作者： Guan, Guiquan;Niu, Qingli;Yang, Jifei;Li, Youquan;Gao, Jinliang;Luo, Jianxun;Yin, Hong

作者机构：

关键词： genomic DNA;fetal bovine serum;Cha1

期刊名称：PARASITOLOGY INTERNATIONAL （ 影响因子：2.23； 五年影响因子：2.281 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A "lewisi-like" Trypanosoma parasite was isolated from the blood of Chinese striped field mice (Apodemus agrarius) trapped in the fields in the Garman Tibet area, Gansu province, China. The parasite was successfully cultivated in vitro in HL-1 medium supplemented 20% fetal bovine serum (FBS). Full formed spheromastigote, metacyclic trypomastigote and trypomastigote structures were all visible in films made from the culture. A nucleotide fragment of 2159-bp length was amplified from genomic DNA of the parasite using specific primers for the 18S rRNA gene of trypanosomes. The alignment indicated that this parasite had higher identities with T. (Herpetosoma) grosi (more than 99.6%) than other Herpetosoma species (less than 98.5%), which suggest that the parasite should be classified as T. (Herpetosoma) grosi. This is the first time in China that an isolation of T. (Herpetosoma) grosi is reported although several strains of T. (Herpetosoma) lewisi have been isolated from rodents of family Muridae in various provinces. Thus, it was designated as T. (Herpetosoma) grosi Chat and deposited in the center of parasite strain collection and preservation in our laboratory for future study. In addition, this culture method will be used to isolate, maintain and study the long-term development of this parasite in vitro

分类号： Q18

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