Isolation and Identification of the DNA Aptamer Target to Acetamiprid

文献类型： 外文期刊

第一作者： He, Jiang

作者： He, Jiang;Liu, Yuan;Liu, Xianjin;He, Jiang;Fan, Mingtao

作者机构：

关键词： Aptamer;pesticide;acetamiprid;SELEX

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： As an alternative to antibodies, aptamers have a great potential as analytical tools for pesticide detection. In this work, aptamers targeting acetamiprid were selected by a specific systematic evolution of ligands by exponential enrichment (SELEX) strategy, where a single-stranded DNA (ssDNA) library was immobilized and target modification was eliminated. After 18 rounds of repeated selection, the ssDNA pool was enriched and then 14 sequences were selected and carefully identified. At last, an acetamiprid-specific aptamer with the apparent dissociation constant (K_d) estimated to be 4.98 μM was successfully obtained. Further work is ongoing to develop an aptamer-based detection method for field determination of this pesticides in agricultural products and environmental samples.

分类号： R15

  • 相关文献

[1]Target site insensitivity mutations in the AChE and LdVssc1 confer resistance to pyrethroids and carbamates in Leptinotarsa decemlineata in northern Xinjiang Uygur autonomous region. Jiang, Wei-Hua,Lu, Wei-Ping,Shi, Xiao-Qin,Xiong, Man-Hui,Wang, Zhi-Tian,Li, Guo-Qing,Guo, Wen-Chao.

[2]Effects of pyrethroids on neuronal excitability of adult honeybees Apis mellifera. Zhang, Yi-Li,Sun, Ji-Hu,Zhou, Ting,Zhou, Wei,Wang, Qiang,Dai, Ping-Li,Liu, Feng,Zhou, Ting,Zhou, Wei,Wang, Qiang,Dai, Ping-Li.

[3]Efficacy of aluminium phosphide as a soil fumigant against nematode and weed in tomato crop. Qiao, Kang,Zhang, Huan,Wang, Kaiyun,Wang, Hongyan,Ji, Xiaoxue.

[4]Generation of Internal-Image Functional Aptamers of Okadaic Acid via Magnetic-Bead SELEX. Lin, Chao,Liu, Zeng-Shan,Li, Lin,Hu, Pan,Gong, Sheng,Li, Yan-Song,Cui, Cheng,Wu, Zong-Cheng,Gao, Yang,Zhou, Yu,Ren, Hong-Lin,Lu, Shi-Ying,Wang, Dong-Xu,Lin, Chao. 2015

[5]The dynamics of dissolved organic N in the fumigated soils. Li, Guitong,Lin, Qimei,Zhao, Xiaorong,Cao, Aocheng.

[6]The combined effect of chemical nematicides and biofumigation on the control of Meloidogyne incognita in glasshouse tomato. Huang, Wen-Kun,Peng, De-Liang,Jiang, Hong-Yun,Long, Hai-Bo,Peng, Huan,Wang, Gao-Feng,Peng, Huan.

[7]Fungal phytotoxins for weed management. Zhang, J. P.,Duan, G. F.,Zhou, Y. J.,Yu, L. Q..

[8]Characterisation of Neonicotinoid and Pymetrozine Resistance in Strains of Bemisia tabaci (Hemiptera: Aleyrodidae) from China. Rao Qiong,Luo Chen,Rao Qiong,Zhang Hong-yu,Jones, Christopher M.,Devine, Greg J.,Gorman, Kevin,Denholm, Ian,Xu Yong-hua. 2012

[9]Sublethal effect of avermectin and acetamiprid on the mortality of different life stages of Brontispa longissima (Gestro) (Coleoptera: Hispidae) and its larvae parasitoid Asecodes hispinarum Boucek (Hymenoptera: Eulophidae). Jin, Tao,Lin, Yu-ying,Jin, Qi-an,Wen, Hai-bo,Peng, Zheng-qiang. 2014

[10]Simultaneous Determination of Plant Growth Regulators and Imidacloprid, Acetamiprid in Tea by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry. Zhu Li,Wang Chen,Chen Hong-Ping,Zhang Ying-Bin,Zhou Su-Juan,Wang Guo-Qing,Liu Xin. 2017

[11]Impact of woodchip biochar amendment on the sorption and dissipation of pesticide acetamiprid in agricultural soils. Yu, Xiang-Yang,Mu, Chang-Li,Liu, Xian-Jin,Gu, Cheng,Liu, Cun.

[12]Dual-competitive lateral flow aptasensor for detection of aflatoxin B-1 in food and feedstuffs. Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yang, Shuming,Chen, Ailiang,Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yang, Shuming,Chen, Ailiang,Huang, Yafei,Ren, Shuyue,Gao, Zhixian. 2018

[13]A Label-Free Aptamer-Based Fluorescent Assay for Cadmium Detection. Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li,Luan, Yunxia,Lu, Anxiang,Chen, Jiayi,Fu, Hailong,Xu, Li. 2016

[14]Aptamer-Based Fluorescence Assay for Hg2+ Determination. Lei Zhao-Jing,Hu Qiu-Hui,Lei Zhao-Jing,Zhang Cun-Zheng,Liu Yuan,Zhang Qiang,Liu Xian-Jin. 2012

[15]Highly Sensitive Colorimetric Detection of Ochratoxin A by a Label-Free Aptamer and Gold Nanoparticles. Luan, Yunxia,Chen, Jiayi,Li, Cheng,Fu, Hailong,Ma, Zhihong,Lu, Anxiang,Luan, Yunxia,Chen, Jiayi,Li, Cheng,Fu, Hailong,Ma, Zhihong,Lu, Anxiang,Xie, Gang. 2015

[16]An oligosorbent-based aptamer affinity column for selective extraction of aflatoxin B-2 prior to HPLC with fluorometric detection. Liu, Hongmei,Luan, Yunxia,Lu, Anxiang,Li, Bingru,Wang, JiHua,Yang, Meihua,Yang, Meihua. 2018

[17]Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles. Luan, Yunxia,Chen, Jiayi,Li, Cheng,Ping, Hua,Ma, Zhihong,Lu, Anxiang,Xie, Gang.

[18]Aptamer based ultrasensitive determination of the beta-adrenergic agonist ractopamine using PicoGreen as a fluorescent DNA probe. Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Zhu, Chao,Zhang, Guilan,Huang, Yafei,Yan, Jiao,Chen, Ailiang,Huang, Yafei,Yan, Jiao.

[19]Split aptamers and their applications in sandwich aptasensors. Chen, Ailiang,Yan, Mengmeng,Yang, Shuming,Chen, Ailiang,Yan, Mengmeng,Yang, Shuming.

[20]A qPCR aptasensor for sensitive detection of aflatoxin M-1. Guo, Xiaodong,Wen, Fang,Zheng, Nan,Li, Songli,Wang, Jiaqi,Guo, Xiaodong,Fauconnier, Marie-Laure,Guo, Xiaodong,Wen, Fang,Zheng, Nan,Li, Songli,Wang, Jiaqi,Zheng, Nan,Wang, Jiaqi.

作者其他论文 更多>>

微信小程序