Molecular cloning and expression analysis of a delta 6-fatty acid desaturase gene from Rhizopus stolonifer strain YF6 which can accumulate high levels of gamma-linolenic acid
文献类型： 外文期刊
第一作者： Wan, Xia
作者： Wan, Xia;Zhang, Yinbo;Wang, Ping;Jiang, Mulan;Wan, Xia;Jiang, Mulan
作者机构：
关键词： desaturase;gamma-linolenic acid;Rhizopus stolonifer
期刊名称：JOURNAL OF MICROBIOLOGY （ 影响因子：3.422； 五年影响因子：3.28 ）
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年卷期：
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收录情况： SCI
摘要： The delta 6-desaturase gene was cloned from Rhizopus stolonifer, which could accumulate up to 49% of gamma-linolenic acid (GLA, C18:3 Δ~(6,9,12)) to the total fatty acids. The cloned DNA contains a 1,380 bp open reading frame encoding a protein of 460 amino acids, which showed high similarity to those of fungal delta 6-desaturases with three conserved histidine-rich motifs and HPGG motif. Notably, this deduced sequence had a shorter C-terminus. Results demonstrated that the cDNA sequence exhibited delta 6-desaturase activity by accumulation of about 22. 4 % of GLA to the total fatty acids in the recombinant Pichia pastoris strain GS115.
分类号： Q93
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