Development of rapid immunoassays for the detection of ractopamine in swine urine

文献类型： 外文期刊

第一作者： Li, X.

作者： Li, X.;Zhang, G.;Deng, R.;Yang, Y.;Liu, Q.;Xiao, Z.;Yang, J.;Xing, G.;Zhao, D.;Cai, S.

作者机构：

关键词： Biochemistry and Molecular Biophysics;Methods and Techniques

期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT （ 影响因子：3.057； 五年影响因子：2.96 ）

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收录情况： SCI

摘要： The monoclonal antibodies (mAbs) against ractopamine (Rac) were prepared and their properties identified by indirect competitive enzyme-linked immunoabsorbant assay (ELISA). The IC50 of mAbs was 2.7 ng ml-1 towards Rac or 9.3 ng ml-1 towards Rac-glucuronides and no cross-reactivity (CR) towards other competitors except dobutamine (CR: 3.76%). Based on the mAbs, the Rac-kit (kit) and Rac-strip (strip) were developed to detect Rac residues in swine urine. The strip and kit assay could be performed within 5-10 min and 2 h, respectively, allowing the analysis of urine samples without the need for sample clean-up. The detection limits were 1 ng ml-1 for kit and 3 ng ml-1 with the unaided eye, and 0.2 ng ml-1 with the Strip Reader for strip. The correlation coefficients (R2) were 0.988 for kit in the range 0-128.0 ng ml-1, and 0.987 for strip in the range 0-10.8 ng ml-1. Comparing the gas chromatography-mass spectrometry (GC-MS) with the kit or strip in swine urine spiked with Rac standards, the differences ranged from 1.4% to 4.5% for kit and 1.0% to 4.7% for strip. However, the differences were greater than 54% for the kit and 55% for the strip test for the analysis of urine from swine treated with Rac. The results obtained from GC-MS using hydrolysed urine samples were generally in good agreement with those obtained from strip or kit using non-hydrolysed urine samples.

分类号： TS2

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