Association analysis of aquaporin 7 (AQP7) gene variants with semen quality and fertility in bulls

文献类型: 外文期刊

第一作者: Ma, Teng-He

作者: Ma, Teng-He;Liu, Ji-Feng;Jiang, Hao;Dai, Li-Sheng;Zhang, Jia-Bao;Ma, Teng-He;Liu, Ji-Feng;Jiang, Hao;Dai, Li-Sheng;Zhao, Zhi-Hui;Zhao, Rui-Feng;Zhao, Yu-Min

作者机构:

关键词: AQP7 gene;single nucleotide polymorphisms;SNPs;association analysis;Simmental bulls;Charolais bulls

期刊名称:TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES ( 影响因子:0.581; 五年影响因子:0.78 )

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收录情况: SCI

摘要: Aquaporin 7 (AQP7) gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms (SNPs) of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A (0.97, 0.96) to B (0.03, 0.04) and C (0.97, 0.91) to D (0.03, 0.09) in Sim-mental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio (P < 0.01) and motility (P < 0.05) in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio (P < 0.05), percentages of viable sperm (P < 0.05), and motility (P < 0.01) in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.

分类号: S85

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