An ISSR-based Approach for the Molecular Detection and Diagnosis of Dwarf Bunt of Wheat, Caused by Tilletia controversa Kuhn

文献类型： 外文期刊

第一作者： Gao, Li

作者： Gao, Li;Chen, Wanquan;Liu, Taiguo

作者机构：

关键词： DNA;molecular marker;intersimple sequence repeat: ISSR;sequence characterized amplified region: SCAR;marker;ISSR818: primer;TCKSF2: primer;TCKSR2: primer;polymorphic pattern

期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.789； 五年影响因子：1.574 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Dwarf bunt of wheat, caused by Tilletia controversa Kuhn, is an important international quarantine disease in many countries. The objective of this investigation was to develop a diagnostic molecular marker generated from intersimple sequence repeat (ISSR) for rapid identification of T. controversa. A total of 60 primers were tested by ISSR to detect DNA polymorphisms between T. controversa and related species. The primer ISSR818 generated a polymorphic pattern displaying a 952- bp DNA fragment specific for T. controversa. The marker was converted into a sequence characterized amplified region (SCAR), and specific primers (TCKSF2/TCKSR2) were designed for use in a PCR detection assay. Its detection limit was 1 ng of DNA, which could be yielded by 1.1 mu g of teliospores in a 25- mu l PCR. Conclusively, a method to distinguish T. controversa from similar pathogenic fungi has been successfully developed based on the use of a SCAR marker.

分类号： Q945

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