Transcriptional regulation of the gene for prothoracicotropic hormone in the silkworm, Bombyx mori

文献类型: 外文期刊

第一作者: Wei, Zhao-Jun

作者: Wei, Zhao-Jun;Yu, Miao;Hong, Gui-Yun;Jiang, Shao-Tong;Tang, Shun-Ming;Yi, Yong-Zhu

作者机构:

关键词: Bombyx mori;prothoracicotropic hormone.;Transcriptional regulation;Promoter;Internet resource.

期刊名称:MOLECULAR BIOLOGY REPORTS ( 影响因子:2.316; 五年影响因子:2.357 )

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收录情况: SCI

摘要: Prothoracicotropic hormone (PTTH) is one of key players in regulation of insect growth, molting, metamorphosis, diapause, and is expressed specifically in the two pairs of lateral PTTH-producing neurosecretory cells in the brain. Analysis of cis-regulatory elements of the PTTH promoter might elucidate the regulatory mechanism controlling PTTH expression. In this study, the PTTH gene promoter of Bombyx mori (Bom-PTTH) was cloned and sequenced. The cis-regulatory elements in Bom-PTTH gene promoter were predicted using Matinspector software, including myocyte-specific enhancer factor 2, pre-B-cell leukemia homeobox 1, TATA box, etc. Transient transfection assays using a series of fragments linked to the luciferase reporter gene indicated that the fragment spanning 110 to +33 bp of the Bom-PTTH promoter showed high ability to support reporter gene expression, but the region of +34 to +192 bp and 512 to 111 bp repressed the promoter activity in the BmN and Bm5 cell lines. Electrophoretic mobility shift assays demonstrated that the nuclear protein could specifically bind to the region spanning 124 to 6 bp of the Bom-PTTH promoter. Furthermore, we observed that the nuclear protein could specifically bind to the 59 to 30 bp region of the Bom-PTTH promoter. A classical TATA box, TATATAA, localized at positions 47 to 41 bp, which is a potential site for interaction with TATA box binding protein (TBP). Mutation of this TATA box resulted in no distinct binding band. Taken together, TATA box was involved in regulation of PTTH gene expression in B. mori.

分类号: Q7

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