Comparison of Five Endogenous Reference Genes for Specific PCR Detection and Quantification of Brassica napus

文献类型： 外文期刊

第一作者： Wu, Gang

作者： Wu, Gang;Zhang, Li;Wu, Yuhua;Cao, Yinglong;Lu, Changming

作者机构：

关键词： Endogenous reference gene;genetically modified organism;rapeseed;real-time PCR;BnACCgB;PEP;FatA;HMG-I/Y;CruA

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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年卷期：

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收录情况： SCI

摘要： Five previously reported Brassica napus endogenous reference genes, including acetyl-CoA carboxylase gene (BnACCgB), phosphoenolpyruvate carboxylase (PEP), oleoyl hydrolase gene (FatA), high-mobility-group protein I/Y gene (HMG-I/Y) and cruciferin A gene (CruA), were analyzed for their PCR specificity between B. napus and other species and the quantification stability among different B. napus cultivars. PCR and sequencing results indicated that none of these systems was species-specific as required by the genetically modified organism labeling policy. When these genes were employed in real-time PCR, BnACCgB and HMG-I/Y systems showed relatively greater heterogeneity among 10 different cultivars. The sequencing results showed that the single nucleotide polymorphism in the primer binding sites was the potential source of the instability in the HMG-I/ Y system. The bias of BnACCg8 was thought to be associated with the inconsistent copy number of this gene.

分类号： R15

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