Comparative transcriptome sequencing analysis to postulate the scheme of regulated leaf coloration in Perilla frutescens

文献类型： 外文期刊

第一作者： Liu, Xiaoning

作者： Liu, Xiaoning;Zhai, Yanning;Liu, Jingyu;Xue, Jingqi;Wang, Shunli;Zhang, Xiuxin;Liu, Jingyu;Markovic, Tatjana

作者机构：

关键词： Perilla; Leaf color; Chlorophyll; Carotenoid; Anthocyanin; Transcriptome sequencing

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：5.1； 五年影响因子：5.3 ）

ISSN： 0167-4412

年卷期： 2023 年 112 卷 3 期

页码：

收录情况： SCI

摘要： Perilla as herb, ornamental, oil and edible plant is widely used in East Asia. Until now, the mechanism of regulated leaf coloration is still unclear. In this study, four different kinds of leaf colors were used to measure pigment contents and do transcriptome sequence to postulate the mechanism of leaf coloration. The measurements of chlorophyll, carotenoid, flavonoid, and anthocyanin showed that higher contents of all the aforementioned four pigments were in full purple leaf 'M357', and they may be determined front and back leaf color formation with purple. Meanwhile, the content of anthocyanin was controlled back leaf coloration. The chromatic aberration analysis and correlative analysis between different pigments and L*a*b* values analysis also suggested front and back leaf color change was correlated with the above four pigments. The genes involved in leaf coloration were identified through transcriptome sequence. The expression levels of chlorophyll synthesis and degradation related genes, carotenoid synthesis related genes and anthocyanin synthesis genes showed up-/down-regulated expression in different color leaves and were consistent of accumulation of these pigments. It was suggested that they were the candidate genes regulated perilla leaf color formation, and genes including F3'H, F3H, F3',5'H, DFR, and ANS are probably important for regulating both front and back leaf purple formation. Transcription factors involved in anthocyanin accumulation, and regulating leaf coloration were also identified. Finally, the probable scheme of regulated both full green and full purple leaf coloration and back leaf coloration was postulated. Key messageThe contents of pigments determined front and back leaf coloration, and the key regulated gene were identified, and the probable scheme of regulated front and back leaf coloration was postulated.

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