Isolation and characterization of a gene encoding a polyethylene glycol-induced cysteine protease in common wheat

文献类型： 外文期刊

第一作者： Zang, Qing-Wei

作者： Zang, Qing-Wei;Wang, Cai-Xiang;Li, Xu-Yan;Guo, Zhi-Ai;Jing, Rui-Lian;Chang, Xiao-Ping;Zhao, Jun

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关键词： complementary DNA: cDNA;cysteine protease: 37353-41-6;polyethylene glycol: PEG;25322-68-3;green fluorescent protein: GFP;abscisic acid: ABA;21293-29-8;transcript: upregulation genome;water stress;drought tolerance;enzyme activity;drought response;gene characterization;gene isolation;water defi cit

期刊名称：JOURNAL OF BIOSCIENCES （ 影响因子：1.826； 五年影响因子：2.107 ）

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收录情况： SCI

摘要： Plant cysteine protease (CP) genes are induced by abiotic stresses such as drought, yet their functions remain largely unknown. We isolated the full-length cDNA encoding a Triticum aestivum CP gene, designated TaCP, from wheat by the rapid amplification of cDNA ends (RACE) method. Sequence analysis revealed that TaCP contains an open reading frame encoding a protein of 362 amino acids, which is 96% identical to barley cysteine protease HvSF42. The TaCP transcript level in wheat seedlings was upregulated during polyethylene glycol (PEG) stress, with a peak appearing around 12 h after treatment. TaCP expression level increased rapidly with NaCl treatment at 48 h. TaCP responded strongly to low temperature (4A degrees C) treatment from 1 h post-treatment and reached a peak of about 40-fold at 72 h. However, it showed only a very slight response to abscisic acid (ABA). More than one copy of TaCP was present in each of the three genomes of hexaploid wheat and its diploid donors. TaCP fused with green fluorescent protein (GFP) was located in the plasma membrane of onion epidermis cells. Transgenic Arabidopsis plants overexpressing TaCP showed stronger drought tolerance and higher CP activity under water-stressed conditions than wild-type Arabidopsis plants. The results suggest that TaCP plays a role in tolerance to water deficit.

分类号： Q

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