Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits

文献类型： 外文期刊

第一作者： Guo, Xiaomin

作者： Guo, Xiaomin;Guo, Junxian;Li, Xiuquan;Yang, Xinming;Li, Lihui

作者机构：

关键词： protein;open reading frame: ORF;PCR primer;high molecular weight glutenin subunits: HMW-GS;functional property;dough strength;wheat quality

期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The high molecular weight glutenin subunits (HMW-GS) can be used for wheat quality improvement. Two novel alleles (designated 1Dx1.5* and 1Dy12.2*, respectively) at the Glu-D1 locus were identified in the Chinese wheat landrace variety 'Jiuquanjinbaoyin' by comparison of subunit mobility with that previously identified in several standard hexaploid wheats. The 1Dx1.5* and 1Dy12.2* genes were isolated using the allele-specific PCR primers and the complete open reading frames (ORFs) were obtained. Allele 1Dx1.5* consists of 2487 bp encoding a mature protein of 827 amino acid residues, whereas 1Dy12.2* consists 1980 bp encoding 658 residues. Comparisons of amino acid sequences analysis showed that 1Dx1.5* had higher similarity with the HMW-GS isolated from the wheat related species (Aegilops tauchi Coss.) than from the bread wheat varieties (Triticum aestivum L.). The 1Dy12.2* amino acid sequence showed a generally similar to the 1Dy12* isolated from Chinese endemic wheats. Meanwhile, the dough properties of the lines expressing (null, 7+8, 1.5*+10), (null, 7+8, 2+12.2*) and (null, 7+8, 2+12), respectively, were measured by a Mixograph, which demonstrates that the alleles 1.5*+10 can be considered as having positive impact on dough strength when compared with the alleles 2+12. In addition, the subunits 2+12.2* also showed a greater impact on dough strength than 2+12.

分类号： S18

  • 相关文献

[1]Characterization of two 1D-encoded omega-gliadin subunits closely related to dough strength and pan bread-making quality in common wheat (Triticum aestivum L.). Wang, Aili,Gao, Liyan,Li, Xiaohui,Zhang, Yanzhen,Yan, Yueming,He, Zhonghu,Xia, Xianchun,Zhang, Yong,He, Zhonghu. 2008

[2]Genetic Variation of High Molecular Weight Glutenin Subunits in Wheat Accessions in China. Guo, Xiaomin,Li, Hongqin,Xiang, Jishan,Xu, Xin,Liu, Weihua,Gao, Ainong,Yang, Xinming,Li, Xiuquan,Li, Lihui,Wang, Ruihui.

[3]Optimisation of production yield by ultrasound-/microwave-assisted acid method and functional property of pectin from sugar beet pulp. Peng, Xiao-Yan,Mu, Tai-Hua,Zhang, Miao,Sun, Hong-Nan,Chen, Jing-Wang,Yu, Ming.

[4]Effects of high pressure on the physicochemical and functional properties of peanut protein isolates. He, Xuan-Hui,Liu, Hong-Zhi,Liu, Li,Zhao, Guan-Li,Wang, Qiang,Chen, Qiong-Ling.

[5]Polyclonal antibodies to grain gliadins and their application in wheat quality prediction. Li, Q. Y.,Song, X. Y.,Zhang, E. Y.,Pei, Y. H.,Li, Q. Y.,Ji, K. M.,Yan, Y. M.. 2008

[6]Research on Development and Experiment of NIR Wheat Quality Quick Detection System. Liu Ling-ling,Zhao Bo,Zhang Yin-qiao,Zhang Xiao-chao. 2013

[7]Sources of the highly expressed wheat bread making (wbm) gene in CIMMYT spring wheat germplasm and its effect on processing and bread-making quality. Guzman, Carlos,Crossa, Jose,Gonzalez-Santoyo, Hector,Huerta, Julio,Singh, Ravi,Dreisigacker, Susanne,Xiao, Yonggui,Huerta, Julio.

[8]Characterization of a Glucose-, Xylose-, Sucrose-, and d-Galactose-Stimulated beta-Glucosidase from the Alkalophilic Bacterium Bacillus halodurans C-125. Xu, Hu,Chen, Jian-Min,Xu, Hu,Xiong, Ai-Sheng,Zhao, Wei,Tian, Yong-Sheng,Peng, Ri-He,Yao, Quan-Hong.

[9]Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides. Wei, You Chuan,Luo, Ting Rong,Wei, You Chuan,Pan, Ting Shuang,Chang, Ming Xian,Huang, Bei,Xu, Zhen,Nie, P.,Pan, Ting Shuang.

[10]Maternal energy and protein affect subsequent growth performance, carcass yield, and meat color in Chinese Yellow broilers. Zhu, C.,Jiang, Z. Y.,Jiang, S. Q.,Zhou, G. L.,Lin, Y. C.,Chen, F.,Hong, P.,Zhu, C.. 2012

[11]Analysis of Spirulina Powder by Fourier Transform Infrared Spectroscopy and Calculation of Protein Content. Liu Hai-jing,Li Wei-ming,Wang Feng,Li An,Zhao Yue-liang,Ha Yi-ming,Liu Hai-jing,Li Wei-ming,Wang Feng,Li An,Zhao Yue-liang,Ha Yi-ming,Xu Chang-hua,Zhou Qun,Sun Su-qin. 2013

[12]Comparative Proteomic Analysis Reveals Differential Root Proteins in Medicago sativa and Medicago truncatula in Response to Salt Stress. Long, Ruicai,Zhang, Tiejun,Kang, Junmei,Cong, Lili,Gao, Yanli,Yang, Qingchuan,Li, Mingna,Sun, Yan,Liu, Fengqi. 2016

[13]Determination of Protein, Fat, Starch, and Amino Acids in Foxtail Millet [Setaria italica (L.) Beauv.] by Fourier Transform Near-Infrared Reflectance Spectroscopy. Yang, Xiu-Shi,Dong, Chuan,Yang, Xiu-Shi,Wang, Li-Li,Zhou, Xian-Rong,Zhu, Zhi-Hua,Li, Nan,Li, Yan,Liu, Fang,Liu, San-Cai,Lu, Ping,Ren, Gui-Xing,Wang, Li-Li,Shuang, Shao-Min. 2013

[14]Protein fractions in barley grains as affected by some agronomic factors and their relationships to malt quality. Wang, J. M.,Chen, J. X.,Dai, F.,Wu, F. B.,Yang, J. M.,Zhang, G. P.. 2007

[15]Effects of Dietary Energy and Protein on Growth Performance and Carcass Quality of Broilers During Finishing Phase. Min, Y. N.,Shi, J. S.,Wei, F. X.,Wang, H. Y.,Hou, X. F.,Niu, Z. . Y.,Liu, F. Z.,Wei, F. X.,Wang, H. Y.,Hou, X. F.. 2012

[16]Genetic diversity analysis of faba bean (Vicia faba L.) germplasms using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Hou, W. W.,Shi, J. B.,Liu, Y. J.,Zhang, X. J.. 2015

[17]Apparent digestibility coefficients of animal and plant feed ingredients for juvenile Pseudobagrus ussuriensis. Che, J.,Su, B.,Tang, B.,Bu, X.,Li, J.,Lin, Y.,Yang, Y.,Ge, X.. 2017

[18]Protein characteristics of Chinese black-grained wheat. Li, WD,Beta, T,Sun, SC,Corke, H. 2006

[19]Proliferation and bone-related gene expression of osteoblasts grown on hydroxyapatite ceramics sintered at different temperature. Wang, CY,Duan, YR,Markovic, B,Barbara, J,Howlett, CR,Zhang, XD,Zreiqat, H. 2004

[20]Growth and survival of tongue sole (Cynoglossus semilaevis Gunther, 1873) larvae fed a compound diet with different protein sources. Chang, Q,Liang, MQ,Wang, JL,Chen, SQ,Zhang, XM,Liu, XD. 2006

作者其他论文 更多>>

微信小程序